Mouse CD321 / JAM1 ELISA Kit

Mouse CD321 / JAM1 ELISA Kit for ELISA and Mouse


Cat No Component Name Package Temp
ARG81857-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81857-002 Standard 2 X 10 ng/vial 4°C
ARG81857-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81857-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81857-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81857-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81857-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81857-008 25X Wash buffer 20 ml 4°C
ARG81857-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81857-010 STOP solution 10 ml (Ready to use) 4°C
ARG81857-011 Plate sealer 4 strips Room temperature


Product Description ARG81857 Mouse CD321 / JAM1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD321 / JAM1 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD321 / JAM1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.2%; Inter-Assay CV: 7.0%
Alternate Names JAM-1; JAM1; Junctional adhesion molecule A; CD antigen CD321; JCAM; JAM-A; Junctional adhesion molecule 1; PAM-1; JAM; JAMA; KAT; Platelet F11 receptor; Platelet adhesion molecule 1; CD321

Application Instructions

Assay Time ~ 5 hours


Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 16456 Mouse F11R

Swiss-port # O88792 Mouse Junctional adhesion molecule A

Gene Symbol F11R
Gene Full Name F11 receptor
Background Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008]
Function Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus. [UniProt]
Highlight Related products:
CD321 antibodies; CD321 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM N-glycosylated. [UniProt]

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  • ARG81857 Mouse CD321 / JAM1 ELISA Kit standard curve image

    ARG81857 Mouse CD321 / JAM1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

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