ARG80200

Mouse IL10 ELISA Kit

Mouse IL10 ELISA Kit for ELISA and Mouse

Cancer kit; Immune System kit
publication_link Publication2

Component

Cat No Component Name Package Temp
ARG80200-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80200-002 Standard (Lyophilized) 3 X 1 ng/vial 4°C
ARG80200-003 Standard diluent buffer 20 ml 4°C
ARG80200-004 Antibody conjugate concentrate 400 µl 4°C
ARG80200-005 Antibody diluent buffer 16 ml 4°C
ARG80200-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80200-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80200-008 20X Wash buffer 50 ml 4°C
ARG80200-009 TMB substrate 12ml 4°C (Protect from light)
ARG80200-010 STOP solution 12ml 4°C
ARG80200-011 Plate sealer 4 strips Room temperature

Overview

Product Description

ARG80200 Mouse IL10 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IL10 in Serum, Plasma, Cell culture supernatants.

Tested Reactivity Ms
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human IL-10, IL-10R;mouse IL-10R;canine IL-10;feline IL-10;porcine IL-10
Target Name IL10
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 15 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 31.25 - 2000 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Full Name interleukin 10
Alternate Names IL10A; GVHDS; TGIF; IL-10; Cytokine synthesis inhibitory factor; CSIF; Interleukin-10

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 16153 Mouse IL10

Swiss-port # P18893 Mouse Interleukin-10

Gene Symbol Il10
Gene Full Name interleukin 10
Background The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. [provided by RefSeq]
Research Area Cancer kit; Immune System kit

Images (1) Click the Picture to Zoom In

  • ARG80200 Mouse IL10 ELISA Kit standard curve image

    ARG80200 Mouse IL10 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

Dietary polysaccharide-rich extract from Eucheuma cottonii modulates the inflammatory response and suppresses colonic injury on dextran sulfate sodium-induced colitis in mice.

ELISA / Mouse

Sudirman S et al.
PLoS One.,  (2018)

publication_link

 

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Red Seaweed Eucheuma cottonii Ethanol Extract Regulates Inflammatory Mediators Production and Prevents Colonic Injury on Acute Colitis Disease in Mice

ELISA / Mouse

Sabri Sudirman et al.
Preprint.,  (2018)

publication_link

 

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Title Download Link
ARG80200 Mouse IL10 ELISA Kit User's manual download icon Download