Mouse OPN / Osteopontin ELISA Kit

Mouse OPN / Osteopontin ELISA Kit for ELISA and Mouse


Cat No Component Name Package Temp
ARG81668-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81668-002 Standard 2 X 10 ng/vial 4°C
ARG81668-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81668-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81668-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81668-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81668-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81668-008 25X Wash buffer 20 ml 4°C
ARG81668-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81668-010 STOP solution 10 ml (Ready to use) 4°C
ARG81668-011 Plate sealer 4 strips Room temperature


Product Description ARG81668 Mouse OPN / Osteopontin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse OPN / Osteopontin in serum, plasma (heparin, EDTA), urine and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name OPN / Osteopontin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA), urine and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.1%; Inter-Assay CV: 7.0%
Alternate Names BSPI; ETA-1; Uropontin; Osteopontin; Nephropontin; SPP-1; Bone sialoprotein 1; BNSP; Urinary stone protein; OPN; Secreted phosphoprotein 1

Application Instructions

Assay Time ~ 5 hours


Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 20750 Mouse SPP1

Swiss-port # P10923 Mouse Osteopontin

Gene Symbol SPP1
Gene Full Name secreted phosphoprotein 1
Background The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Function Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.

Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. [UniProt]
Highlight Related products:
OPN antibodies; OPN ELISA Kits;
Related news:
Detecting MMPs and their non-ECM substrates
The role of HDGF in tumor angiogenesis
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.

N- and O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG81668 Mouse OPN / Osteopontin ELISA Kit standard curve image

    ARG81668 Mouse OPN / Osteopontin ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81668 Mouse Osteopontin ELISA Kit User manual download icon Download