Mouse/Rat Insulin (Ultra sensitive) ELISA Kit

Mouse/Rat Insulin (Ultra sensitive) ELISA Kit for ELISA and Mouse,Rat

publication_link Publication1


Product Description

ARG81295 Mouse/Rat Insulin (Ultra sensitive) ELISA Kit is an ultra sensitive Enzyme Immunoassay kit for the quantification of Mouse/Rat Insulin in serum, plasma and cell culture supernatants.

Tested Reactivity Ms, Rat
Tested Application ELISA
Specificity Mouse / Rat Insulin: 100%
Hamster Insulin: Detected
Rat C-peptide, Rat pancreatic polypeptide, Porcine glucagon, Human Insulin like growth factor-I and II: Not Detected
Target Name Insulin
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm.

Sensitivity 50 pg/ml (5 µl sample volume); 5 pg/ml (100 µl sample volume)
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 0.1 - 6.4 ng/ml
Sample Volume 5 µl or 100 µl
Alternate Names IDDM; IDDM2; IDDM1; ILPR; MODY10; Insulin; IRDN; Insulin-2; InsII; Ins-2

Application Instructions

Assay Time ~ 3 hours


Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 16334 Mouse INS

Swiss-port # P01326 Mouse Insulin

Gene Symbol Ins2
Gene Full Name insulin II
Background After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
Function Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. [UniProt]
Highlight Related products:
Insulin antibodies; Insulin ELISA Kits; Insulin Duos / Panels; Insulin recombinant proteins;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

Images (1) Click the Picture to Zoom In

  • ARG81295 Mouse Insulin (Ultra sensitive) ELISA Kit standard curve image

    ARG81295 Mouse Insulin (Ultra sensitive) ELISA Kit results of a typical standard run with optical density reading at 450 - 630 nm.

Specific References

GLP-1/GLP-1R Signaling Regulates Ovarian PCOS-Associated Granulosa Cells Proliferation and Antiapoptosis by Modification of Forkhead Box Protein O1 Phosphorylation Sites.

ELISA / Mouse / Serum

Sun Zhihua et al.
Int J Endocrinol.,  (2020)



Title Download Link
ARG81295 Mouse/Rat Insulin (Ultra sensitive) ELISA Kit User's manual download icon Download