ARG53954

Mouse anti-Human Lambda Light Chain antibody [4C2] (APC)

Mouse anti-Human Lambda Light Chain antibody [4C2] (APC) for Flow cytometry and Human

Immune System antibody

Overview

Product Description APC-conjugated Mouse Monoclonal antibody [4C2] recognizes Human Lambda Light Chain
Tested Reactivity Hu
Species Does Not React With Goat, Gpig, Hm, Rb, Sheep
Tested Application FACS
Host Mouse
Clonality Monoclonal
Clone 4C2
Isotype IgG1
Target Name Lambda Light Chain
Antigen Species Human
Target Ig Human Lambda light chains
Conjugation APC
Alternate Names IGL@; IGLC6

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IGL
Gene Full Name immunoglobulin lambda locus
Background Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two classes of light chains, kappa and lambda. This region represents the germline organization of the lambda light chain locus. The locus includes V (variable), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. Several V segments and three C segments are known to be incapable of encoding a protein and are considered pseudogenes. The locus also includes several non-immunoglobulin genes, many of which are pseudogenes or are predicted by automated computational analysis or homology to other species. [provided by RefSeq, Jul 2008]
Research Area Immune System antibody

Images (3) Click the Picture to Zoom In

  • ARG53954 Mouse anti-Human Lambda Light Chain antibody [4C2] (APC) FACS image

    Flow Cytometry: Human peripheral whole blood stained with ARG53954 Mouse anti-Human Lambda Light Chain antibody [4C2] (APC) (10 µl reagent / 100 µl of peripheral whole blood).

  • ARG53954 Mouse anti-Human Lambda Light Chain antibody [4C2] (APC) FACS image

    Flow Cytometry: Human peripheral blood stained with ARG53954 Mouse anti-Human Lambda Light Chain antibody [4C2] (APC).

  • ARG53954 Mouse anti-Human Lambda Light Chain antibody [4C2] (APC) FACS image

    Flow Cytometry: Separation of Human Lambda Light Chain positive B-Lymphocytes (red) from Human Lambda Light Chain negative B-Lymphocytes (black-dashed). Human peripheral whole blood stained with ARG53954 Mouse anti-Human Lambda Light Chain antibody [4C2] (APC) (10 µl reagent / 100 µl of peripheral whole blood).