ARG30008

Neuron Development Marker Antibody Duo (Nestin, Tuj1)

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG52345 anti-Nestin antibody [4D11] Mouse mAb Hu, Ms, Rat ICC/IF, IHC-Fr, WB 50 μl
ARG65321 anti-beta III Tubulin antibody Goat pAb Hu WB 50 μg

Overview

Product Description

During embryonic neurogenesis, neural progenitors give rise to intermediate progenitors, before they are further differentiated into premature neurons. In the telencephalon, neural progenitor cells express Nestin and losses its expression soon after they committed their fate to becoming neurons. Premature neurons express TujI/TUBB3, which has been commonly used as a marker to identify neural differentiation in the developing brain or even in stem cell studies.
arigo grouped Nestin and TujI as control duo to offer reliable antibodies for the distinction of neural progenitors and premature neurons during the development of the central nervous system.

Properties

Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Highlight Related news:
Neuronal Development Marker
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody

Images (7) Click the Picture to Zoom In

  • ARG52345 anti-Nestin antibody [4D11] ICC/IF image

    Immunofluorescence: Cultured neonatal Rat neurons and glia showing nestin labeling in red (ARG52345 Nestin antibody [4D11]) and vimentin (ARG52468 anti-Vimentin antibody) in green.

    Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.

  • ARG52345 anti-Nestin antibody [4D11] ICC/IF image

    Immunofluorescence: Cortical neuron-glial cells from E20 Rat stained with ARG52345 anti-Nestin antibody [4D11] (red) at 1:500 dilution and costained with ARG52328 anti-MAP2 antibody (green) at 1:5000 dilution. Hoechst (blue) for nuclear staining.

    The Nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.

  • ARG52345 anti-Nestin antibody [4D11] WB image

    Western Blot: neonatal rat brain lysate stained with Nestin antibody (ARG52345) showing specific immunolabeling of the ~220-240 kDa nestin doublet.

  • ARG65321 anti-TUBB3 antibody WB image

    Western Blot: HepG2 lysate (35 µg protein in RIPA buffer) stained with ARG65321 anti-Tubulin beta III antibody at 2 µg/ml dilution.

  • ARG52345 anti-Nestin antibody [4D11] WB image

    Western blot: Rat brain (P18) homogenate stained with ARG52345 anti-Nestin antibody [4D11].

  • ARG52345 anti-Nestin antibody [4D11] WB image

    Western blot: Rat brain (embryonic, E18), C6 and SH-SY5Y cell lysates stained with ARG52345 anti-Nestin antibody [4D11] (green) at 1:500 dilution.

  • ARG52345 anti-Nestin antibody [4D11] ICC/IF image

    Immunofluorescence: cultured neonatal rat neurons and glia showing nestin labeling in red (ARG52345 Nestin antibody [4D11]) and vimentin (ARG52468 anti-Vimentin antibody) in green. Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.