ARG30038
Phospho Akt Antibody Duo (pS473 pT308)
Cancer antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
Publication14
Component
| Cat No | Component Name | Host clonality | Reactivity | Application | Package |
|---|---|---|---|---|---|
| ARG51558 | anti-Akt phospho (Ser473) antibody | Rabbit pAb | Hu, Ms, Rat | ICC/IF, IHC-P, WB | 50 μl |
| ARG51559 | anti-Akt phospho (Thr308) antibody | Rabbit pAb | Hu, Ms, Rat | ICC/IF, IHC-P, WB | 50 μl |
Overview
| Product Description | Akt, also known as Protein kinase B (PKB), is a serine/threonine-specific protein kinase that plays a key role in multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription and cell migration. AKT can be phosphorylated by 3-phosphoinositide dependent protein kinase 1 (PDK1) at threonine 308 (Thr308).In the other hand, serine 473 (Ser473) is phosphorylated by a the mTOR complex 2 (mTORC2) and DNA-dependent protein kinase (DNA-PK). AKT activity is dependent on the phosphorylation of Thr308 or Ser473 residues. ARG30038 and ARG30039 AKT phospho Duos, including antibodies recognize total Akt protein or phosphorylated Akt at Ser473 or Thr308, are designed for the scientists to study Akt regulation signaling. |
|---|---|
| Target Name | Akt |
| Alternate Names | Phospho Akt antibody; Akt phospho (Ser473) antibody; Akt phospho (Thr308) antibody |
Properties
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
|---|---|
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | Antibody Duo for Phospho Akt (pS473 pT308) |
|---|---|
| Research Area | Cancer antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
Images (19) Click the Picture to Zoom In
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ARG51558 anti-Akt phospho (Ser473) antibody IHC-P image
Immunohistochemistry: Mouse xenograft tumor stained with ARG22104 anti-Ly6G + Ly6C antibody [RB6-8C5] , ARG40666 anti-mTOR phospho (Ser2448) antibody , ARG51558 anti-Akt phospho (Ser473) antibodyand ARG62605 anti-PCNA antibody [PC10] .
From Wu LH et al. J Cancer (2022), doi: 10.7150/jca.75163, Fig. 5. D.
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ARG51559 anti-Akt phospho (Thr308) antibody WB image
Western blot: HCT15 and SW620 stained with ARG51559 anti-Akt phospho (Thr308) antibody.
From Wang Q et al. Int J Med Sci (2019), doi: 10.7150/ijms.38439, Fig. 3D.
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ARG51558 anti-Akt phospho (Ser473) antibody WB image
Western blot: 30 µg of HeLa cells untreated or treated with EGF. Lysates stained with ARG51558 anti-Akt phospho (Ser473) antibody at 1:500 dilution.
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ARG51558 anti-Akt phospho (Ser473) antibody ICC-IF image
Immunofluorescence: Rat neurons stained with ARG51558 anti-Akt phospho (Ser473) antibody.
From Gao Z et al. Neuroreport- (2021), doi: 10.1097/WNR.0000000000001593, Fig. 4.
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ARG51559 anti-Akt phospho (Thr308) antibody WB image
Western blot: Rat heart stained with ARG51559 anti-Akt phospho (Thr308) antibody.
From Wang Q et al. Mol Med Rep (2019), doi: 10.3892/mmr.2018.9681, Fig. 5A.
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ARG51559 anti-Akt phospho (Thr308) antibody WB image
Western blot: Mouse wound stained with ARG51559 anti-Akt phospho (Thr308) antibody.
From Hao M et al. J Inflamm Res. (2022), doi: 10.3389/fphar.2022.913408, Fig. 6. B.
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ARG51558 anti-Akt phospho (Ser473) antibody WB image
Western Blot: extracts from 293 cells, treatedwith EGF or calf intestinal phosphatase (CIP), stained with anti-Akt (phospho Ser473) antibody ARG51558.
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ARG51558 anti-Akt phospho (Ser473) antibody WB image
Western Blot: extracts from SK-BR-3 cells,treated with insulin and EGF, and pretreated with U0126and LY294002 cells stained with anti-Akt (phospho Ser473) antibody ARG51558.
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ARG51559 anti-Akt phospho (Thr308) antibody WB image
Western Blot: extracts from HeLa cells untreated or treated with EGF, serum or both stained with anti-Akt (phospho Thr308) antibody ARG51559.
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ARG51558 anti-Akt phospho (Ser473) antibody WB image
Western blot: HepG2 stained with ARG51558 anti-Akt phospho (Ser473) antibody and ARG51712 anti-mTOR phospho (Ser2448) antibody.
From Lu J et al. Toxicology- (2021), doi: 10.1016/j.tox.2021.152716, Fig. 2. A.
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ARG51558 anti-Akt phospho (Ser473) antibody WB image
Western blot: Rat neurons stained with ARG51558 anti-Akt phospho (Ser473) antibody.
From Gao Z et al. Neuroreport- (2021), doi: 10.1097/WNR.0000000000001593, Fig. 3. A.
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ARG51558 anti-Akt phospho (Ser473) antibody IHC-P image
Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-Akt (phospho Ser473) antibody ARG51558 (left) or the same antibody preincubated with blocking peptide (right).
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ARG51558 anti-Akt phospho (Ser473) antibody IHC-P image
Immunohistochemistry: paraffinembeddedhuman Lung carcinoma tissue stained with anti-Akt (phospho Ser473) antibody ARG51558.
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ARG51559 anti-Akt phospho (Thr308) antibody IHC-P image
Immunohistochemistry: paraffin-embedded human lung carcinoma tissue, stained with anti-Akt (phospho Thr308) antibody ARG51559 (left) or the same antibody preincubated with blocking peptide (right).
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ARG51559 anti-Akt phospho (Thr308) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells showing nuclear dot staining stained with anti-Akt (phospho Thr308) antibody ARG51559.
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ARG51559 anti-Akt phospho (Thr308) antibody IHC-P image
Immunohistochemistry: paraffin-embedded human lung carcinoma tissue, stained with anti-Akt (phospho Thr308) antibody ARG51559.
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ARG51559 anti-Akt phospho (Thr308) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells showing nuclear dot staining stained with anti-Akt (phospho Thr308) antibody ARG51559.
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ARG51558 anti-Akt phospho (Ser473) antibody WB image
Western blot: PC12 stained with ARG51558 anti-Akt phospho (Ser473) antibody.
From Liu Y et al. Pharmaceuticals (Basel). (2024), doi: 10.3390/ph17070972, Fig. 4. A.
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ARG51558 anti-Akt phospho (Ser473) antibody WB image
Western blot: Mouse heart stained with ARG51558 anti-Akt phospho (Ser473) antibody and ARG56418 anti-Akt (pan) antibody.
From Lin QY et al. Am J Physiol Heart Circ Physiol- (2019), doi: 10.1152/ajpheart.00566.2019, Fig. 3. D.
Specific References
Sika Deer Velvet Antler Peptide Exerts Neuroprotective Effect in a Parkinson's Disease Model via Regulating Oxidative Damage and Gut Microbiota
ARG51558: WB / Mouse
Downregulation of AKT/mTOR signaling pathway for Salmonella-mediated autophagy in human anaplastic thyroid cancer
ARG51558: IHC-P / Mouse
Chitosan/Sodium Alginate/Velvet Antler Blood Peptides Hydrogel Promoted Wound Healing by Regulating PI3K/AKT/mTOR and SIRT1/NF-κB Pathways
ARG51559: WB / Mouse
Chitosan/Sodium Alginate/Velvet Antler Blood Peptides Hydrogel Promotes Diabetic Wound Healing via Regulating Angiogenesis, Inflammatory Response and Skin Flora
ARG51559: WB / Mouse
Dopamine D2 receptor regulates cortical synaptic pruning in rodents
ARG51558: WB / Rat
Astilbin ameliorates oxidative stress and apoptosis in D-galactose-induced senescence by regulating the PI3K/Akt/m-TOR signaling pathway in the brains of mice
ARG51559: WB / Mouse
1,3-dichloro-2-propanol induced lipid accumulation by blocking autophagy flux in HepG2 cells.
ARG51558: WB / Human
Mild hypothermia protects rat cortical neurons against oxygen-glucose deprivation/reoxygenation injury via the PI3K/Akt pathway.
ARG51558: WB, ICC/IF / Rat
Pharmacological blockage of ICAM-1 improves angiotensin II-induced cardiac remodeling by inhibiting adhesion of LFA-1+ monocytes.
ARG51558: WB / Mouse
SSRP1 influences colorectal cancer cell growth and apoptosis via the AKT pathway
ARG51559: WB / Human
Function of BRD4 in the pathogenesis of high glucose‑induced cardiac hypertrophy.
ARG51559: WB / Rat
Urea transport B gene induces melanoma B16 cell death via activation of p53 and mitochondrial apoptosis.
ARG51559: WB / Mouse
Protective effects of dioscin against fructose-induced renal damage via adjusting Sirt3-mediatedoxidative stress, fibrosis, lipid metabolism and inflammation.
ARG51558: WB / Rat
电针对控制性超排卵小鼠子宫内膜磷酸化胰岛素样生长因子-1受体表达及其信号转导通路的调控
ARG51558: WB / Mouse
