ARG30025

Phospho FAK Antibody Duo (pY576/577, pY925)

Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG51624 anti-FAK phospho (Tyr925) antibody Rabbit pAb Hu, Ms, Rat WB, ICC/IF 50 μl
ARG51831 anti-FAK phospho (Tyr576 / Tyr577) antibody Rabbit pAb Hu, Ms, Rat WB, ICC/IF 50 μl

Overview

Product Description

Focal Adhesion Kinase (FAK), also known as PTK2 protein tyrosine kinase 2 (PTK2), is a non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis.

Increased levels of FAK and Src proteins found in tumors and the importance of the FAK-Src signaling complex to tumor cell behavior, in addition, phosphorylation of FAK Tyr925 is a Src-dependent phosphorylation. Therefore, suggests that phosphorylation of Tyr925 on FAK may provide a useful indicator of increased signaling through the Src-FAK signaling complex in tumors. On the other hand, a RET-FAK transactivation mechanism consisting of direct phosphorylation of FAK Tyr-576/577 by RET but not Tyr925 and a reciprocal phosphorylation of RET by FAK.

To study the correction of FAK and your protein of interest, ARG30025 FAK phospho Duos provides antibodies to detect the phosphorylated-FAK at Tyr576/577 or Tyr925 for the user in FAK study.

Target Name FAK
Alternate Names Phospho FAK antibody; Phospho Focal adhesion kinase antibody; FAK phospho (Tyr925) antibody; FAK phospho (Tyr576 / Tyr577) antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Phospho Focal adhesion kinase (FAK) Antibody Duo (pY576/577, pY925)
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody

Images (4) Click the Picture to Zoom In

  • ARG51624 anti-FAK phospho (Tyr925) antibody WB image

    Western Blot: extracts from 293 cells stained with anti-FAK (phospho Tyr925) antibody ARG51624 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).

  • ARG51831 anti-FAK phospho (Tyr576 Tyr577) antibody WB image

    Western Blot: extracts from HT29 cells untreated or treated with PMA stained with anti-FAK (phospho Tyr576 Tyr577) antibody ARG51831.

  • ARG51624 anti-FAK phospho (Tyr925) antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with anti-FAK (phospho Tyr925) antibody ARG51624.

  • ARG51831 anti-FAK phospho (Tyr576 Tyr577) antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with anti-FAK (phospho Tyr576 Tyr577) antibody ARG51831.