ARG30052

Phospho mTOR Antibody Duo (Total, pS2448)

Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody
publication_link Publication2

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG54675 anti-mTOR antibody Rabbit pAb Hu, Ms ELISA, ICC/IF, WB 25 μg
ARG51712 anti-mTOR phospho (Ser2448) antibody Rabbit pAb Hu, Ms, Rat ICC/IF, IHC-P, WB 50 μl

Overview

Product Description

The mammalian target of rapamycin (mTOR) regulates cell growth with the growth factor and nutrient status of the cell. mTOR is a direct substrate for the AKT kinase that phosphorylates mTOR at Ser-2448. Later it was found that the phosphorylation of mTOR at Ser-2448 also requires autophosphorylation at Ser-2481.



mTOR phospho duo allows scientists to study the function of mTOR in the cell proliferation and homeostasis abnormality in tumor cells. The control of mTOR pSer-2448 phosphorylation by insulin might give more insight into the potential of mTOR as a target for cancer therapy.

Target Name mTOR
Alternate Names Phospho mTOR antibody; Phospho mechanistic target of rapamycin antibody; mTOR phospho (Ser2448) antibody; mTOR antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Phospho mechanistic target of rapamycin (mTOR) Antibody Duo (Total, pS2448)
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody

Images (7) Click the Picture to Zoom In

  • ARG51712 anti-mTOR phospho (Ser2448) antibody WB image

    Western Blot: extracts from HeLa cells, treatedwith IFNa or calf intestinal phosphatase (CIP), stained with anti-mTOR (phospho Ser2448) antibody ARG51712.

  • ARG51712 anti-mTOR phospho (Ser2448) antibody WB image

    Western Blot: extracts from 293 cells untreatedor treated with PMA stained with anti-mTOR (phospho Ser2448) antibody ARG51712.

  • ARG51712 anti-mTOR phospho (Ser2448) antibody IHC-P image

    Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-mTOR (phospho Ser2448) antibody ARG51712 (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51712 anti-mTOR phospho (Ser2448) antibody ICC/IF image

    Immunofluorescence: methanol-fixed MCF cells stained with anti-mTOR (phospho Ser2448) antibody ARG51712.

  • ARG51712 anti-mTOR phospho (Ser2448) antibody IHC-P image

    Immunohistochemistry: paraffin-embedded human breast carcinomatissue stained with ARG51712 anti-mTOR (phospho Ser2448) antibody.

  • ARG54675 anti-mTOR antibody ICC/IF image

    Immunocytochemistry: L1210 cells stained with ARG54675 anti-mTOR antibody at 2 μg/ml.

  • ARG54675 anti-mTOR antibody WB image

    Western blot: L1210 cell lysate stained with ARG54675 anti-mTOR antibody at (A) 1 and (B) 2 μg/ml.

Specific References

Astilbin ameliorates oxidative stress and apoptosis in D-galactose-induced senescence by regulating the PI3K/Akt/m-TOR signaling pathway in the brains of mice

ARG51712: WB / Mouse

Yue Zhang et al.
Int Immunopharmacol.,  (2021)

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1,3-dichloro-2-propanol induced lipid accumulation by blocking autophagy flux in HepG2 cells.

ARG51712: WB / Human

Jing Lu et al.
Toxicology.,  (2021)

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