ARG30272

Phospho p38 MAPK Antibody Duo (Total, pThr180/Tyr182)

publication_link Publication10

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG55258 anti-p38 MAPK antibody Rabbit pAb Hu, Ms, Rat ICC/IF, WB 50 μl
ARG51850 anti-p38 MAPK phospho (Thr180 / Tyr182) antibody Rabbit pAb Hu, Ms, Rat WB 50 μl

Overview

Product Description

p38 MAP kinase (MAPK), also called RK or CSBP, is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress. Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors. MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2 , Max, and MEF2.

Rouse, J. et al. (1994) Cell 78, 1027-37.
Han, J. et al. (1994) Science 265, 808-11.
Lee, J.C. et al. (1994) Nature 372, 739-46.
Freshney, N.W. et al. (1994) Cell 78, 1039-49.
Raingeaud, J. et al. (1995) J Biol Chem 270, 7420-6.
Zervos, A.S. et al. (1995) Proc Natl Acad Sci U S A 92, 10531-4.
Yang, S.H. et al. (1999) Mol Cell Biol 19, 4028-38

Target Name p38 MAPK
Alternate Names Phospho p38 MAPK antibody; Phospho Mitogen-activated protein kinase 14 antibody; p38 MAPK phospho (Thr180 / Tyr182) antibody; p38 MAPK antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (5) Click the Picture to Zoom In

  • ARG30272 Phospho-p38 MAPK Ab Duo (Total, pThr180/Tyr182) WB image

    Western blot: 30 µg of HeLa treated with TNFa at 0, 10, 20, and 30 min. The blots stained with ARG51850 anti-P38 MAPK phospho (Thr180/Tyr182) antibody, ARG55258 anti-p38 MAPK antibody, and ARG65714 anti-Vinculin antibody.

  • ARG51850 anti-P38 MAPK phospho (Thr180/Tyr182) antibody WB image

    Western Blot: extracts from HeLa cells untreated or treated with TNFa+CA stained with anti-P38 MAPK (phospho Thr180/Tyr182) antibody ARG51850.

  • ARG55258 anti-p38 MAPK antibody ICC/IF image

    Immunofluorescence: A549 cell stained with ARG55258 anti-p38 MAPK antibody. Blue: DAPI for nuclear staining.

  • ARG55258 anti-p38 / MAPK14 antibody ICC/IF image

    Immunofluorescence: A549 cell stained with ARG55258 anti-p38 / MAPK14 antibody. Blue: DAPI for nuclear staining.

  • ARG55258 anti-p38 MAPK antibody WB image

    Western blot: 30 µg of HeLa, and Mouse liver lysate stained with ARG55258 anti-p38 MAPK antibody at 1:2000 dilution.

Specific References

DHPA Protects SH-SY5Y Cells from Oxidative Stress-Induced Apoptosis via Mitochondria Apoptosis and the Keap1/Nrf2/HO-1 Signaling Pathway

ARG51850: WB / Human

Yunhui Cai  et al.
Antioxidants (Basel),  (2022)

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Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions

ARG51850, ARG55258: WB / Human

Peng-Hsiang Fang et al.
Molecular Medicine,  (2022)

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Angiotensin II Induces Cardiac Edema and Hypertrophic Remodeling through Lymphatic-Dependent Mechanisms

ARG51850: WB / Mouse ARG55258: WB / Mouse

Jie Bai et al.
Oxid Med Cell Longev.,  (2022)

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Shikonin Induced Program Cell Death through Generation of Reactive Oxygen Species in Renal Cancer Cells

ARG51850: WB / Human

Ming-Feng Tsai et al.
Antioxidants (Basel).,  (2021)

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Angiotensin-(1-7) treatment blocks lipopolysaccharide-induced organ damage, platelet dysfunction, and IL-6 and nitric oxide production in rats.

ARG51850: WB / Rat

Hsin-Jung Tsai et al.
Sci Rep.,  (2021)

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Inhibition of inflammatory response by α-lipoic acid in autoimmune thyroiditis mice.

ARG55258: WB / Mouse

Mayinu Yusufu et al.
Basic & Clinical Medicine.,  (2021)

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Discoidin Domain Receptor 1 Regulates Runx2 during Osteogenesis of Osteoblasts and Promotes Bone Ossification via Phosphorylation of p38.

ARG55258: WB / Mouse

Liang-Yin Chou et al.
Int J Mol Sci.,  (2020)

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Polyphenols from Toona sinensiss Seeds Alleviate Neuroinflammation Induced by 6-Hydroxydopamine Through Suppressing p38 MAPK Signaling Pathway in a Rat Model of Parkinson's Disease.

ARG55258: WB, ICC/IF / Rat

Zhuang Wenxin et al.
Neurochem Res.,  (2020)

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The Different Effects of IFN-β and IFN-γ on the Tumor-Suppressive Activity of Human Amniotic Fluid-Derived Mesenchymal Stem Cells.

ARG51850: WB / Human

Du Jingchun et al.
Stem Cells International.,  (2019)

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Indirubin Inhibits LPS-Induced Inflammation via TLR4 Abrogation Mediated by the NF-kB and MAPK Signaling Pathways.

ARG55258: WB / Mouse

Lai JL et al.
Inflammation.,  (2016)

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