ARG70313

Rat IFN gamma recombinant protein (Fc-His-tagged, C-ter)

Rat IFN gamma recombinant protein (Fc-His-tagged, C-ter) for SDS-PAGE,Binding Activity and Rat

Overview

Product Description HEK293 expressed, Fc-His-tagged (C-ter) Rat IFN gamma recombinant protein.
Tested Reactivity Rat
Tested Application Binding, SDS-PAGE
Target Name IFN gamma
Species Rat
A.A. Sequnce Gln23 - Cys156 of Rat IFN gamma (NP_620235.1) with an Fc-6X His tag at the C-terminus.
Expression System HEK293
Alternate Names IFN-gamma; Interferon gamma; Immune interferon; IFG; IFI

Application Instructions

Application Note Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized recombinant Human IFN-gamma R1 at 3µg/ml (100µl/well) can bind recombinant Rat IFNG, the EC50 of Rat IFNG is 1 µg/ml.

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 92% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IFNG
Gene Full Name interferon, gamma
Background This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]
Function Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. [UniProt]
Cellular Localization Secreted. [UniProt]
Calculated MW 19 kDa
PTM Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. [UniProt]

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  • ARG70313 Rat IFN gamma recombinant protein (Fc-His-tagged, C-ter) SDS-PAGE image

    SDS-PAGE analysis of ARG70313 Rat IFN gamma recombinant protein (Fc-His-tagged, C-ter).