ARG20701

Rat IgG2a Isotype Control antibody [KLH/G2a-1-1]

Rat IgG2a Isotype Control antibody [KLH/G2a-1-1] for Blocking,ELISA,Flow cytometry,FLISA,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation

Overview

Product Description Rat Monoclonal antibody [KLH/G2a-1-1] as a negative control antibody for Rat IgG2a
Tested Application BL, ELISA, FACS, FLISA, IHC-Fr, IHC-P, IP
Specificity KLH
Host Rat
Clonality Monoclonal
Clone KLH/G2a-1-1
Isotype IgG2a, kappa
Target Name Rat IgG2a
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
ELISAAssay-dependent
FACSAssay-dependent
FLISAAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer BBS (pH 8.2)
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (2) Click the Picture to Zoom In

  • ARG20701 Rat IgG2a Isotype Control antibody [KLH/G2a-1-1] ELISA image

    ELISA: The plate was coated with serially diluted ARG20701 Rat IgG2a Isotype Control antibody [KLH/G2a-1-1]. Immunoglobulin was detected with Mouse anti-Rat IgG1 antibody (Biotin), ARG21703 Mouse anti-Rat IgG2a antibody [2A8F4] (Biotin), ARG21710 Mouse anti-Rat IgG2b antibody [2B10A8] (Biotin-maleimide), ARG21715 Mouse anti-Rat IgG2c antibody [2C8F1] (Biotin), and ARG23837 Mouse anti-Rat IgM antibody [M2A1] (Biotin) followed by ARG23912 Streptavidin (HRP) and quantified.

  • ARG20701 Rat IgG2a Isotype Control antibody [KLH/G2a-1-1] control image

    Control: Frozen section of BALB/c Mouse intestine tissue stained with ARG20701 Rat IgG2a Isotype Control antibody [KLH/G2a-1-1] (left) and ARG21041 anti-CD104 / Integrin beta 4 antibody [346-11A] (right), followed by ARG23770 Goat anti-Rat IgG (H+L) antibody (Biotin) (pre-adsorbed), Streptavidin (FITC) and DAPI.