ARG70218

SARS-CoV-2 Spike recombinant protein (S1 Subunit) (His-tagged, C-ter)

SARS-CoV-2 Spike recombinant protein (S1 Subunit) (His-tagged, C-ter) for SDS-PAGE and Virus

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Overview

Product Description E. coli expressed, His-tagged (C-ter) SARS-CoV-2 Spike recombinant protein (S1 Subunit).
Tested Reactivity Virus
Tested Application SDS-PAGE
Target Name SARS-CoV-2 Spike protein (S1 Subunit)
Species Virus
A.A. Sequnce MVNLT TRTQL PPAYT NSFTR GVYYP DKVFR SSVLH STQDL FLPFF SNVTW FHAIH VSGTN GTKRF DNPVL PFNDG VYFAS TEKSN IIRGW IFGTT LDSKT QSLLI VNNAT NVVIK VCEFQ FCNDP FLGVY YHKNN KSWME SEFRV YSSAN NCTFE YVSQP FLMDL EGKQG NFKNL REFVF KNIDG YFKIY SKHTP INLVR DLPQG FSALE PLVDL PIGIN ITRFQ TLLAL HRSYL TPGDS SSGWT AGAAA YYVGY LQPRT FLLKY NENGT ITDAV DCALD PLSET KCTLK SFTVE KGIYQ TSNFR VQPTE SIVRF PNITN LCPFG EVFNA TRFAS VYAWN RKRIS NCVAD YSVLY NSASF STFKC YGVSP TKLND LCFTN VYADS FVIRG DEVRQ IAPGQ TGKIA DYNYK LPDDF TGCVI AWNSN NLDSK VGGNY NYLYR LFRKS NLKPF ERDIS TEIYQ AGSTP CNGVE GFNCY FPLQS YGFQP TNGVG YQPYR VVVLS FELLH APATV CGPKK STNLV KNKCV NFNFN GLTGT GVLTE SNKKF LPFQQ FGRDI ADTTD AVRDP QTLEI LDITP CSFGG VSVIT PGTNT SNQVA VLYQD VNCTE VPVAI HADQL TPTWR VYSTG SNVFQ TRAGC LIGAE HVNNS YECDI PIGAG IC with polyhistidine tag at the C-terminus.
Expression System E. coli

Properties

Form Powder
Purification Note Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 98% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

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Images (1) Click the Picture to Zoom In

  • ARG70218 SARS-CoV-2 Spike recombinant protein (S1 Subunit) (His-tagged, C-ter) SDS-PAGE image

    SDS-PAGE analysis of ARG70218 SARS-CoV-2 Spike recombinant protein (S1 Subunit) (His-tagged, C-ter).

Specific References

Immune-Mediated Inflammatory Responses of Alveolar Epithelial Cells: Implications for COVID-19 Lung Pathology

Functional assay / Virus

Amelia Barilli et al.
Biomedicines,  (2022)

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SARS-CoV-2 Spike protein is not pro-inflammatory in human primary macrophages: endotoxin contamination and lack of protein glycosylation as possible confounders

Functional Assay / Virus

Gloria Cinquegrani et al.
Cell Biol Toxicol.,  (2022)

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Standardized Extract of Asparagus officinalis Stem Attenuates SARS-CoV-2 Spike Protein-Induced IL-6 and IL-1β Production by Suppressing p44/42 MAPK and Akt Phosphorylation in Murine Primary Macrophages

Functional assay / Virus

Ken Shirato et al.
Molecules,  (2021)

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Endothelial Cell Activation by SARS-CoV-2 Spike S1 Protein: A Crosstalk between Endothelium and Innate Immune Cells

Stimulation  / 

Bianca Maria Rotoli et al.
Biomedicines.,  (2021)

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ETAS®50 Attenuates SARS-CoV-2 Spike Protein-Induced IL-6 and IL-1β Production by Suppressing p44/42 MAPK and Akt Phosphorylation in Murine Primary Macrophages.

Ken Shirato et al.
Preprint.,  (2021)

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SARS-CoV-2 spike protein S1 subunit induces pro-inflammatory responses via toll-like receptor 4 signaling in murine and human macrophages.

Stimulation / 

Ken Shirato et al.
Heliyon.,  (2021)

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