ARG10690

anti-14-3-3 eta antibody [3G12]

anti-14-3-3 eta antibody [3G12] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow,Pig

Overview

Product Description Mouse Monoclonal antibody [3G12] recognizes 14-3-3 eta
Tested Reactivity Hu, Ms, Rat, Cow, Pig
Predict Reactivity Chk
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 3G12
Isotype IgG1
Target Name 14-3-3 eta
Antigen Species Human
Immunogen Full length Human recombinant 14-3-3 eta protein.
Conjugation Un-conjugated
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
Alternate Names YWHA1; 14-3-3 protein eta; Protein AS1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:500 - 1:1000
IHC-Fr1:500 - 1:1000
WB1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 22629 Mouse YWHAH

GeneID: 25576 Rat YWHAH

GeneID: 7533 Human YWHAH

Gene Symbol YWHAH
Gene Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
Background This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009]
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1. [UniProt]
PTM Phosphorylated on Ser-59 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.

Images (3) Click the Picture to Zoom In

  • ARG10690 anti-14-3-3 eta antibody [3G12] ICC/IF image

    Immunocytochemistry: Rat mixed neuron / glial cell cultures stained with ARG10690 anti-14-3-3 eta antibody [3G12] (red). Neuronal perikarya are very rich in 14-3-3η which has a diffused cytoplasmic staining pattern. The blue is DAPI staining of nuclear DNA.

  • ARG10690 anti-14-3-3 eta antibody [3G12] ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG10690 anti-14-3-3 eta antibody [3G12] (red) at 1:1000 dilution. DAPI (blue) for nuclear staining.

    Clone 3G12 reveals the diffuse cytoplasmic distribution of 14-3-3 eta protein with higher concentration in the perinuclear region.

  • ARG10690 anti-14-3-3 eta antibody [3G12] WB image

    Western blot: 1) protein standard, 2) Rat whole brain, 3) Mouse whole brain, 4) NIH/3T3, 5) Hek293, 6) HeLa, 7) SH-SY5Y, and 8) C6 cells stained with ARG10690 anti-14-3-3 eta antibody [3G12].