ARG54732

anti-14-3-3 gamma antibody

anti-14-3-3 gamma antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Cancer antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
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Overview

Product Description Rabbit Polyclonal antibody recognizes 14-3-3 gamma
Tested Reactivity Hu, Ms
Predict Reactivity Bov, Rat, Chk, Xenopus
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Target Name 14-3-3 gamma
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 136-164 (Center) of Human 14-3-3 gamma (NP_036611.2).
Conjugation Un-conjugated
Alternate Names 14-3-3 protein gamma; PPP1R170; 14-3-3GAMMA; KCIP-1; Protein kinase C inhibitor protein 1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse cerebellum

Properties

Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22628 Mouse YWHAG

GeneID: 7532 Human YWHAG

Swiss-port # P61981 Human 14-3-3 protein gamma

Swiss-port # P61982 Mouse 14-3-3 protein gamma

Gene Symbol YWHAG
Gene Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma
Background This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. [From Uniprot]
Cellular Localization Cytoplasm.
Research Area Cancer antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 28 kDa
PTM Phosphorylated by various PKC isozymes.

Images (3) Click the Picture to Zoom In

  • ARG54732 anti-14-3-3 gamma antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colon carcinoma stained with ARG54732 anti-14-3-3 gamma antibody.

  • ARG54732 anti-14-3-3 gamma antibody WB image

    Western blot: 35 µg of Mouse cerebellum lysate stained with ARG54732 anti-14-3-3 gamma antibody.

  • ARG54732 anti-14-3-3 gamma antibody FACS image

    Flow Cytometry: WiDr cells stained with ARG54732 anti-14-3-3 gamma antibody (bottom histogram) or without primary antibody control (top histogram).

Customer's Feedback

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Review for anti-14-3-3 gamma antibody

Application:WB

Sample:HeLa

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC