ARG52215

anti-14-3-3 phospho (Ser58) antibody

anti-14-3-3 phospho (Ser58) antibody for Immunohistochemistry,Western blot and Human,Rat

Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes 14-3-3 phospho (Ser58)
Tested Reactivity Hu, Rat
Predict Reactivity Ms, Bov, Chk, Dog, NHuPrm, Sheep, Xenopus laevis, Zfsh
Tested Application IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name 14-3-3
Antigen Species Rat
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 conjugated to KLH
Conjugation Un-conjugated
Alternate Names Protein 1054; 14-3-3 protein beta/alpha; Protein kinase C inhibitor protein 1; HS1; GW128; KCIP-1; YWHAA; HEL-S-1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC1:500
WB1:1000
Application Note Specific for the ~29k 14-3-3 protein phosphorylated at Ser58. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 56011 Rat YWHAB

GeneID: 7529 Human YWHAB

Swiss-port # P31946 Human 14-3-3 protein beta/alpha

Swiss-port # P35213 Rat 14-3-3 protein beta/alpha

Gene Symbol YWHAB
Gene Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta
Background 14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. These functions of 14-3-3 proteins are facilitated by, if not dependent on, its dimeric structure. Recent work has demonstrated that the dimeric status of the 14-3-3 protein is regulated by site-specific serine phosphorylation (Woodcock et al., 2003).
Research Area Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 28 kDa
PTM The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.

Images (1) Click the Picture to Zoom In

  • ARG52215 anti-14-3-3 phospho (Ser58) antibody WB image

    Western blot: Rat brainstem lysate showing phospho-specific immunolabeling of the ~29k 14-3-3 protein phosphorylated at Ser 58 stained with ARG52215 anti-14-3-3 phospho (Ser58) antibody.