ARG51169

anti-14-3-3 zeta antibody

anti-14-3-3 zeta antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes 14-3-3 zeta
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name 14-3-3 zeta
Antigen Species Human
Immunogen Peptide sequence around aa.56~60 (R-S-S-W-R) derived from Human 14-3-3 zeta.
Conjugation Un-conjugated
Alternate Names HEL4; 14-3-3-zeta; Protein kinase C inhibitor protein 1; YWHAD; KCIP-1; HEL-S-3; 14-3-3 protein zeta/delta

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22631 Mouse YWHAZ

GeneID: 25578 Rat YWHAZ

GeneID: 7534 Human YWHAZ

Gene Symbol YWHAZ
Gene Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta
Background This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. [UniProt]
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Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 28 kDa
PTM The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Thr-232; inhibits binding of RAF1. Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53.

Images (2) Click the Picture to Zoom In

  • ARG51169 anti-14-3-3 zeta antibody WB image

    Western Blot: extracts from HepG2, HeLa and 293 cells stained with anti-14-3-3 zeta antibody ARG51169.

  • ARG51169 anti-14-3-3 zeta antibody IHC-P image

    Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-14-3-3 zeta antibody ARG51169 (left) or the same antibody preincubated with blocking peptide (right).