anti-4E-BP1 phospho (Thr37 / Thr46) antibody

anti-4E-BP1 phospho (Thr37 / Thr46) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Gene Regulation antibody; Metabolism antibody


Product Description Rabbit Polyclonal antibody recognizes 4E-BP1 phospho (Thr37 / Thr46)
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Specificity This antibody detects 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name 4E-BP1
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of threonine 46 (S-T-T(p)-P-G) derived from Human 4E-BP1.
Conjugation Un-conjugated
Alternate Names Phosphorylated heat- and acid-stable protein regulated by insulin 1; eIF4E-binding protein 1; PHAS-I; Eukaryotic translation initiation factor 4E-binding protein 1; 4EBP1; 4E-BP1; BP-1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 116636 Rat EIF4EBP1

GeneID: 13685 Mouse EIF4EBP1

GeneID: 1978 Human EIF4EBP1

Gene Symbol EIF4EBP1
Gene Full Name eukaryotic translation initiation factor 4E binding protein 1
Background 4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Function Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. [UniProt]
Research Area Gene Regulation antibody; Metabolism antibody
Calculated MW 13 kDa
PTM Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70, corresponding to the hyperphosphorylated form, is regulated by mTORC1 and abolishes binding to EIF4E.
Ubiquitinated: when eIF4E levels are low, hypophosphorylated form is ubiquitinated by the BCR(KLHL25) complex, leading to its degradation and serving as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. Not ubiquitinated when hyperphosphorylated (at Thr-37, Thr-46, Ser-65 and Thr-70) or associated with eIF4E.

Images (2) Click the Picture to Zoom In

  • ARG51714 anti-4E-BP1 phospho (Thr37 / Thr46) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51714 anti-4E-BP1 phospho (Thr37 / Thr46) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51714 anti-4E-BP1 phospho (Thr37 / Thr46) antibody WB image

    Western blot: Extracts from HeLa cells stained with ARG51714 anti-4E-BP1 phospho (Thr37 / Thr46) antibody and the same antibody preincubated with blocking peptide.