ARG66331

anti-6X His tag antibody [SQab1879]

anti-6X His tag antibody [SQab1879] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Other

Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab1879] recognizes 6X His tag
Tested Reactivity Other
Tested Application FACS, ICC/IF, IP, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1879
Isotype IgG
Target Name 6X His tag
Antigen Species Others
Immunogen KLH-conjugated HHHHHH (6X His).
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:200 - 1:1000
ICC/IF1:800 - 1:2000
IP1:50
WB1:1000 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (6) Click the Picture to Zoom In

  • ARG66331 anti-6X His tag antibody [SQab1879] ICC/IF image

    Immunofluorescence: 293 cells trasnfected with N-terminal 6X His tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66331 anti-6X His tag antibody [SQab1879] (green) at 1:10,000 and 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG66331 anti-6X His tag antibody [SQab1879] FACS image

    Flow Cytometry: 293 cells transfected with N-terminal (blue) and C-terminal (red) 6X His tagged gene. The cells were stained with ARG66331 anti-6X His tag antibody [SQab1879] at 1:1,000 dilution in 1x PBS/1% BSA for 30 min at at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.

  • ARG66331 anti-6X His tag antibody [SQab1879] WB image

    Western blot: 20 µg of 1) fusion protein with N-terminal 6X His tag, 2) fusion protein with C-terminal 6X His tag, 3) Multi-tag fusion protein containing 6X His tag, 4) 293 cells lysate-transfected with N-terminal 6X His tagged gene, 5) 293 cells lysate-transfected with C-terminal 6X His tagged gene, 6) fusion protein with N-terminal 6X His tag, 7) fusion protein with C-terminal 6X His tag, and 8) untransfected 293 cell lysate stained with ARG66331 anti-6X His tag antibody [SQab1879] at 1:2000 dilution.

  • ARG66331 anti-6X His tag antibody [SQab1879] IP image

    Immunoprecipitation: 0.05 mg of 293 whole cells lysate transfected with N-terminal 6X His tagged gene immunoprecipitated (1:50) and stained with ARG66331 anti-6X His tag antibody [SQab1879]. 1) ARG66331 IP in 293 whole cell lysate transfected with N-terminal 6X His tagged gene, 2) PBS instead of ARG66331 in 293 whole cell lysate transfected with N-terminal 6X His tagged gene, and 3) 293 whole cell lysate transfected with N-terminal 6X His tagged gene, 10 µg (input).

  • ARG66331 anti-6X His tag antibody [SQab1879] ICC/IF image

    Immunofluorescence: 293 cells trasnfected with C-terminal 6X His tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66331 anti-6X His tag antibody [SQab1879] (green) at 1:800 and 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG66331 anti-6X His tag antibody [SQab1879] IP image

    Immunoprecipitation: 0.2 mg of 293 whole cells lysate transfected with C-terminal 6X His tagged gene immunoprecipitated (1:50) and stained with ARG66331 anti-6X His tag antibody [SQab1879]. 1) ARG66331 IP in 293 whole cells lysate transfected with C-terminal 6X His tagged gene, 2) PBS instead of ARG66331 in 293 whole cells lysate transfected with C-terminal 6X His tagged gene, and 3) 293 whole cells lysate transfected with C-terminal 6X His tagged gene, 10 µg (input).