ARG21511

anti-8 Hydroxyguanosine (8-OHdG) antibody [15A3]

anti-8 Hydroxyguanosine (8-OHdG) antibody [15A3] for Dot blot,ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and Other

Overview

Product Description

Mouse Monoclonal antibody [15A3] recognizes 8 Hydroxyguanosine (8-OHdG)

Tested Reactivity Other
Tested Application Dot, ELISA, ICC/IF, IHC-Fr, IHC-P
Specificity Recognizes markers of oxidative damage to DNA (8- hydroxy-2' -deoxyguanosine, 8- hydroxyguanine and 8- hydroxyguanosine)
Host Mouse
Clonality Monoclonal
Clone 15A3
Isotype IgG2b
Target Name 8 Hydroxyguanosine (8-OHdG)
Immunogen 8-hydroxy-guanosine-BSA and -casein conjugates
Conjugation Un-conjugated
Alternate Names 8-Hydroxy Guanine; 8-OH-dG; 8OHG; 80G; 8 hydroxyguanine; 8 hydroxy 2' deoxyguanosine; 8 hydroxyguanosine; 8 OHG; 8-OHG; 8OHdG

Application Instructions

Application Suggestion
Tested Application Dilution
DotAssay-dependent
ELISAAssay-dependent
ICC/IFAssay-dependent
IHC-Fr1:1000
IHC-P1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.1% Sodium azide and 50% Glycerol
Preservative 0.1% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background 8-hydroxyguanine, 8-hydroxy-2’-deoxyguanonsine and 8- hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2’-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations
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Images (2) Click the Picture to Zoom In

  • ARG21511 anti-8 Hydroxyguanosine (8-OHdG) antibody [15A3] IHC image

    Immunohistochemistry: Formalin-fixed Mouse inflamed colon stained with ARG21511 anti-8 Hydroxyguanosine (8-OHdG) antibody [15A3] at 1:1,000,000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 min at RT. Magnification: 40x.

  • ARG21511 anti-8 Hydroxyguanosine (8-OHdG) antibody [15A3] IHC-P image

    Immunohistochemistry: Bouin's fixed and paraffin-embedded Mouse backskin stained with ARG21511 anti-8 Hydroxyguanosine (8-OHdG) antibody [15A3] at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat anti-Mouse (green) at 1:50 for 1 hour at RT.