anti-8 Hydroxyguanosine (8-OHdG) antibody [15A3]
anti-8 Hydroxyguanosine (8-OHdG) antibody [15A3] for Dot blot,ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and Other
Mouse Monoclonal antibody [15A3] recognizes 8 Hydroxyguanosine (8-OHdG)
|Tested Application||Dot, ELISA, ICC/IF, IHC-Fr, IHC-P|
|Specificity||Recognizes markers of oxidative damage to DNA (8- hydroxy-2' -deoxyguanosine, 8- hydroxyguanine and 8- hydroxyguanosine)|
|Target Name||8 Hydroxyguanosine (8-OHdG)|
|Immunogen||8-hydroxy-guanosine-BSA and -casein conjugates|
|Alternate Names||8-Hydroxy Guanine; 8-OH-dG; 8OHG; 80G; 8 hydroxyguanine; 8 hydroxy 2' deoxyguanosine; 8 hydroxyguanosine; 8 OHG; 8-OHG; 8OHdG|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Purification||Purification with Protein G.|
|Buffer||PBS, 0.1% Sodium azide and 50% Glycerol|
|Preservative||0.1% Sodium azide|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Background||8-hydroxyguanine, 8-hydroxy-2’-deoxyguanonsine and 8- hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2’-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations|
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Images (2) Click the Picture to Zoom In
ARG21511 anti-8 Hydroxyguanosine (8-OHdG) antibody [15A3] IHC image
Immunohistochemistry: Formalin-fixed Mouse inflamed colon stained with ARG21511 anti-8 Hydroxyguanosine (8-OHdG) antibody [15A3] at 1:1,000,000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 min at RT. Magnification: 40x.
ARG21511 anti-8 Hydroxyguanosine (8-OHdG) antibody [15A3] IHC-P image
Immunohistochemistry: Bouin's fixed and paraffin-embedded Mouse backskin stained with ARG21511 anti-8 Hydroxyguanosine (8-OHdG) antibody [15A3] at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat anti-Mouse (green) at 1:50 for 1 hour at RT.