ARG45593
anti-A RAF antibody
anti-A RAF antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes A RAF |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | A RAF |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human A RAF. |
| Conjugation | Un-conjugated |
| Alternate Names | ARAF; A-Raf Proto-Oncogene, Serine/Threonine Kinase; A-Raf; ARAF1; V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog 1; Serine/Threonine-Protein Kinase A-Raf; Proto-Oncogene A-Raf-1; Proto-Oncogene Pks; EC 2.7.11.1; PKS2; V-Raf Murine Sarcoma 3611 Viral Oncogene-Like Protein; A-Raf Proto-Oncogene Serine/Threonine-Protein Kinase; Ras-Binding Protein DA-Raf; Proto-Oncogene A-Raf; Oncogene ARAF1; EC 2.7.11; RAFA1; PKS |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 68 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ARAF |
| Gene Full Name | A-Raf Proto-Oncogene, Serine/Threonine Kinase |
| Background | Enables protein serine/threonine kinase activity. Involved in negative regulation of apoptotic process; regulation of TOR signaling; and regulation of protein metabolic process. Predicted to be active in cytosol and mitochondrion. Biomarker of high grade glioma. [provided by Alliance of Genome Resources, Feb 2025] |
| Function | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. Phosphorylates PFKFB2. [UniProt] |
| Cellular Localization | Cytosol; Mitochondrion. [UniProt] |
| Calculated MW | 68 kDa |
| PTM | Phosphoprotein. [UniProt] |
Images (5) Click the Picture to Zoom In
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ARG45593 anti-A RAF antibody ICC/IF image
Immunofluorescence: MCF-7 stained with ARG45593 anti-A RAF antibody at 5 μg/ml dilution.
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ARG45593 anti-A RAF antibody WB image
Western blot: Hela and MCF-7 stained with ARG45593 anti-A RAF antibody at 0.5 μg/ml dilution.
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ARG45593 anti-A RAF antibody FACS image
Flow Cytometry: SiHa stained with ARG45593 anti-A RAF antibody at 1 µg/10^6 cells dilution.
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ARG45593 anti-A RAF antibody WB image
Western blot: Rat testis stained with ARG45593 anti-A RAF antibody at 0.5 μg/ml dilution.
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ARG45593 anti-A RAF antibody WB image
Western blot: Mouse testis stained with ARG45593 anti-A RAF antibody at 0.5 μg/ml dilution.
