anti-AATF phospho (Ser477) antibody

anti-AATF phospho (Ser477) antibody for Western blot and Human

Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody


Product Description Rabbit Polyclonal antibody recognizes AATF phospho (Ser477)
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Bov, Dog, NHuPrm, Sheep, Xenopus laevis
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name AATF
Antigen Species Human
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser477 conjugated to KLH
Conjugation Un-conjugated
Alternate Names BFR2; Apoptosis-antagonizing transcription factor; CHE1; CHE-1; Rb-binding protein Che-1; Protein AATF; DED

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note Specific for the ~66k Che-1 protein phosphorylated at Ser477. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide that was used to geneRate the antibody but not by the corresponding dephospho-peptide.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 26574 Human AATF

Swiss-port # Q9NY61 Human Protein AATF

Gene Symbol AATF
Gene Full Name apoptosis antagonizing transcription factor
Background Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression (Burgdorf et al., 2004). It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species (Xie et al., 2004). The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage (Bruno et al., 2006).
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody
Calculated MW 63 kDa
PTM Hyperphosphorylated during the G1/S phase transition.

Images (1) Click the Picture to Zoom In

  • ARG52244 anti-AATF phospho (Ser477) antibody WB image

    Western blot: HeLa lysate showing specific immunolabeling of the ~66k Che-1 protein phosphorylated at Ser 477 stained with ARG52244 anti-AATF phospho (Ser477) antibody.
    The phosphospecificity is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen.