ARG45488
anti-ACADS antibody
anti-ACADS antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ACADS |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ACADS |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to C-terminal region of human ACADS. |
| Conjugation | Un-conjugated |
| Alternate Names | EC 1.3.8.1; Butyryl-CoA dehydrogenase; ACAD3; SCAD; Short-chain specific acyl-CoA dehydrogenase, mitochondrial |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 44 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACADS |
| Gene Full Name | acyl-CoA dehydrogenase, C-2 to C-3 short chain |
| Background | This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms. [provided by RefSeq, Oct 2014] |
| Function | Short-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (By similarity). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (By similarity). Among the different mitochondrial acyl-CoA dehydrogenases, short-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 4 to 6 carbons long primary chains (PubMed:21237683, PubMed:11134486). [UniProt] |
| Cellular Localization | Mitochondrion matrix. [UniProt]. [UniProt] |
| Calculated MW | 44 kDa |
| PTM | Acetylation; Phosphoprotein. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG45488 anti-ACADS antibody IHC-P image
Immunohistochemistry: Human colon cancer stained with ARG45488 anti-ACADS antibody at 2 μg/ml dilution.
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ARG45488 anti-ACADS antibody ICC/IF image
Immunofluorescence: A549 stained with ARG45488 anti-ACADS antibody at 5 μg/ml dilution.
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ARG45488 anti-ACADS antibody WB image
Western blot: HepG2 stained with ARG45488 anti-ACADS antibody at 0.5 μg/ml dilution.
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ARG45488 anti-ACADS antibody FACS image
Flow Cytometry: HepG2 stained with ARG45488 anti-ACADS antibody at 1 µg/10^6 cells dilution.
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ARG45488 anti-ACADS antibody WB image
Western blot: Rat kidney stained with ARG45488 anti-ACADS antibody at 0.5 μg/ml dilution.
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ARG45488 anti-ACADS antibody WB image
Western blot: Mouse kidney stained with ARG45488 anti-ACADS antibody at 0.5 μg/ml dilution.
