anti-ACER2 antibody

anti-ACER2 antibody for Western blot and Human,Rat

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Product Description Rabbit Polyclonal antibody recognizes ACER2
Tested Reactivity Hu, Rat
Predict Reactivity Ms, Mk
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ACER2
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide within the center region of Human ACER2.
Conjugation Un-conjugated
Alternate Names AlkCDase 2; Acylsphingosine deacylase 3-like; N-acylsphingosine amidohydrolase 3-like; EC; ASAH3L; ALKCDase2; Alkaline ceramidase 2; Alkaline CDase 2; haCER2

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 32 kDa


Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol.
Preservative 0.01% Sodium azide
Stabilizer 30% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 340485 Human ACER2

Swiss-port # Q5QJU3 Human Alkaline ceramidase 2

Gene Symbol ACER2
Gene Full Name alkaline ceramidase 2
Background The sphingolipid metabolite sphingosine-1-phosphate promotes cell proliferation and survival, whereas its precursor, sphingosine, has the opposite effect. The ceramidase ACER2 hydrolyzes very long chain ceramides to generate sphingosine (Xu et al., 2006 [PubMed 16940153]). [supplied by OMIM, Jul 2010]
Function Golgi ceramidase that catalyzes the hydrolysis of ceramides into sphingoid bases like sphingosine and free fatty acids at alkaline pH (PubMed:16940153, PubMed:18945876, PubMed:20207939, PubMed:20089856). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed:20207939). Has a better catalytic efficiency towards unsaturated long-chain ceramides, including C18:1-, C20:1- and C24:1-ceramides (PubMed:16940153, PubMed:18945876, PubMed:20207939, PubMed:20089856). Saturated long-chain ceramides and unsaturated very long-chain ceramides are also good substrates, whereas saturated very long-chain ceramides and short-chain ceramides are poor substrates (PubMed:20089856). Also hydrolyzes dihydroceramides to produce dihydrosphingosine (PubMed:20207939, PubMed:20628055). It is the ceramidase that controls the levels of circulating sphingosine-1-phosphate and dihydrosphingosine-1-phosphate in plasma through their production by hematopoietic cells (By similarity). Regulates cell proliferation, autophagy and apoptosis by the production of sphingosine and sphingosine-1-phosphate (PubMed:16940153, PubMed:26943039, PubMed:28294157, PubMed:29229990). As part of a p53/TP53-dependent pathway, promotes for instance autophagy and apoptosis in response to DNA damage (PubMed:26943039, PubMed:28294157, PubMed:29229990). Through the production of sphingosine, may also regulate the function of the Golgi complex and regulate the glycosylation of proteins (PubMed:18945876). [UniProt]
Cellular Localization Golgi apparatus membrane; Multi-pass membrane protein. [UniProt]
Calculated MW 31 kDa

Images (1) Click the Picture to Zoom In

  • ARG66642 anti-ACER2 antibody WB image

    Western blot: MCF7 and PC12 whole cell lysates stained with ARG66642 anti-ACER2 antibody.

Specific References

TIMELESS regulates sphingolipid metabolism and tumor cell growth through Sp1/ACER2/S1P axis in ER-positive breast cancer.

WB / Human

Shan Zhang et al.
Cell Death Dis.,  (2020)