ARG45496
anti-ACO1 / Aconitase 1 antibody
anti-ACO1 / Aconitase 1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Monkey
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ACO1 / Aconitase 1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mk |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ACO1 / Aconitase 1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human ACO1 / Aconitase 1. |
| Conjugation | Un-conjugated |
| Alternate Names | ACO; Aconitate hydratase 1; Aconitase 1; Citrate hydro-lyase 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 98 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.01% Sodium azide and 4% Trehalose. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACO1 |
| Gene Full Name | aconitase 1 |
| Background | Encodes an aconitase that can catalyze the conversion of citrate to isocitrate through a cis-aconitate intermediate, indicating that it may participate in the TCA cycle and other primary metabolic pathways. The protein is believed to accumulate in the mitochondria and the cytosol. It affects CSD2 (At2g28190 - a superoxide dismutase) transcript levels and may play a role in the response to oxidative stress. This enzyme can also specifically bind to the 5' UTR of CSD2 in vitro. |
| Function | Catalyzes the isomerization of citrate to isocitrate via cis-aconitate; Contributes to oxidative stress tolerance. [UniProt] |
| Cellular Localization | Cytoplasm; Mitochondrion. [UniProt] |
| Calculated MW | 98 kDa |
| PTM | Bifunctional iron sensor that switches between 2 activities depending on iron availability. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG45496 anti-ACO1 / Aconitase1 antibody ICC/IF image
Immunofluorescence: A431 stained with ARG45496 anti-ACO1 / Aconitase1 antibody at 4 μg/ml dilution.
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ARG45496 anti-ACO1 / Aconitase1 antibody WB image
Western blot: Monkey liver stained with ARG45496 anti-ACO1 / Aconitase1 antibody at 0.5 μg/ml dilution.
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ARG45496 anti-ACO1 / Aconitase1 antibody FACS image
Flow Cytometry: SiHa stained with ARG45496 anti-ACO1 / Aconitase1 antibody at 1 µg/10^6 cells dilution.
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ARG45496 anti-ACO1 / Aconitase1 antibody IHC-P image
Immunohistochemistry: Human rectal cancer stained with ARG45496 anti-ACO1 / Aconitase1 antibody at 1 μg/ml dilution.
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ARG45496 anti-ACO1 / Aconitase1 antibody WB image
Western blot: Rat liver stained with ARG45496 anti-ACO1 / Aconitase1 antibody at 0.5 μg/ml dilution.
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ARG45496 anti-ACO1 / Aconitase1 antibody WB image
Western blot: Mouse liver stained with ARG45496 anti-ACO1 / Aconitase1 antibody at 0.5 μg/ml dilution.
