ARG57994
anti-ACSL1 antibody
anti-ACSL1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ACSL1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ACSL1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around aa. 604-698 of Human ACSL1. |
| Conjugation | Un-conjugated |
| Alternate Names | LACS 2; Long-chain acyl-CoA synthetase 2; LACS1; ACS1; LACS2; Long-chain-fatty-acid--CoA ligase 1; Long-chain acyl-CoA synthetase 1; FACL2; FACL1; LACS; EC 6.2.1.3; LACS 1; Long-chain fatty acid-CoA ligase 2; Acyl-CoA synthetase 1; Palmitoyl-CoA ligase 1; Palmitoyl-CoA ligase 2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 80 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.025% Sodium azide and 2.5% BSA. |
| Preservative | 0.025% Sodium azide |
| Stabilizer | 2.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACSL1 |
| Gene Full Name | acyl-CoA synthetase long-chain family member 1 |
| Background | The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013] |
| Function | Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate. [UniProt] |
| Calculated MW | 78 kDa |
Images (4) Click the Picture to Zoom In
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ARG57994 anti-ACSL1 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human liver cancer tissue stained with ARG57994 anti-ACSL1 antibody at 1 µg/ml dilution. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) for 20 min.
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ARG57994 anti-ACSL1 antibody WB image
Western blot: Rat liver, Mouse HEPA and A549 lysate stained with ARG57994 anti-ACSL1 antibody at 0.5 µg/ml dilution.
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ARG57994 anti-ACSL1 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse kidney tissue stained with ARG57994 anti-ACSL1 antibody at 1 µg/ml dilution. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) for 20 min.
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ARG57994 anti-ACSL1 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Rat kidney tissue stained with ARG57994 anti-ACSL1 antibody at 1 µg/ml dilution. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) for 20 min.
