anti-ADAM17 / TACE antibody
anti-ADAM17 / TACE antibody for Western blot,ICC/IF and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
|Product Description||Rabbit Polyclonal antibody recognizes ADAM17 / TACE|
|Tested Application||ICC/IF, WB|
|Specificity||This antibody recognizes human, mouse, and rat TACE, and 80-130kDa bands are detected in immunoblots. These bands may represent mature protein, precursor, and glycosylated TACE.|
|Target Name||ADAM17 / TACE|
|Immunogen||Peptide corresponding to aa 807-823 of human TACE (accession no. NP_003174). This sequence differs from mouse and rat TACE by one amino acid.|
|Alternate Names||CD antigen CD156b; TNF-alpha convertase; NISBD; ADAM 17; Disintegrin and metalloproteinase domain-containing protein 17; Snake venom-like protease; TACE; NISBD1; ADAM18; EC 188.8.131.52; CSVP; CD156B; TNF-alpha-converting enzyme|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Positive Control||HeLa and Jurkat|
|Buffer||PBS (pH 7.4) and 0.02% Sodium azide|
|Preservative||0.02% Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||ADAM metallopeptidase domain 17|
|Background||TNF is synthesized as a 26kDa type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17kDa mature TNF . TNF converting enzyme (TACE) has been identified, and human and mouse TACE cDNAs have been cloned. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGF , L-selectin, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and mouse tissues.|
|Function||Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2. [UniProt]|
|Research Area||Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody|
|Calculated MW||93 kDa|
|PTM||The precursor is cleaved by a furin endopeptidase.
Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791. Phosphorylation at THR-735 by MAPK14 is required for ADAM17-mediated ectodomain shedding.
Images (2) Click the Picture to Zoom In
ARG54323 anti-ADAM17 / TACE antibody WB image
Western blot: 1:HeLa; 2:Jurkat; 3:HeLa in the presence of blocking peptide; 4:Jurkat in the presence of blocking peptide stained with ARG54323 anti-ADAM17 / TACE antibody at 1 μg/ml dilution.
ARG54323 anti-ADAM17 / TACE antibody ICC/IF image
Immunofluorescence: HeLa stained with ARG54323 anti-ADAM17 / TACE antibody at 10 μg/ml dilution.