ARG54323

anti-ADAM17 / TACE antibody

anti-ADAM17 / TACE antibody for Western blot,ICC/IF and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes ADAM17 / TACE
Tested Reactivity Hu
Tested Application ICC/IF, WB
Specificity This antibody recognizes human, mouse, and rat TACE, and 80-130kDa bands are detected in immunoblots. These bands may represent mature protein, precursor, and glycosylated TACE.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ADAM17 / TACE
Antigen Species Human
Immunogen Peptide corresponding to aa 807-823 of human TACE (accession no. NP_003174). This sequence differs from mouse and rat TACE by one amino acid.
Conjugation Un-conjugated
Alternate Names CD antigen CD156b; TNF-alpha convertase; NISBD; ADAM 17; Disintegrin and metalloproteinase domain-containing protein 17; Snake venom-like protease; TACE; NISBD1; ADAM18; EC 3.4.24.86; CSVP; CD156B; TNF-alpha-converting enzyme

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa and Jurkat

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6868 Human ADAM17

Swiss-port # P78536 Human Disintegrin and metalloproteinase domain-containing protein 17

Gene Symbol ADAM17
Gene Full Name ADAM metallopeptidase domain 17
Background TNF is synthesized as a 26kDa type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17kDa mature TNF . TNF converting enzyme (TACE) has been identified, and human and mouse TACE cDNAs have been cloned. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGF , L-selectin, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and mouse tissues.
Function Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 93 kDa
PTM The precursor is cleaved by a furin endopeptidase.
Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791. Phosphorylation at THR-735 by MAPK14 is required for ADAM17-mediated ectodomain shedding.

Images (2) Click the Picture to Zoom In

  • ARG54323 anti-ADAM17 / TACE antibody WB image

    Western blot: 1:HeLa; 2:Jurkat; 3:HeLa in the presence of blocking peptide; 4:Jurkat in the presence of blocking peptide stained with ARG54323 anti-ADAM17 / TACE antibody at 1 μg/ml dilution.

  • ARG54323 anti-ADAM17 / TACE antibody ICC/IF image

    Immunofluorescence: HeLa stained with ARG54323 anti-ADAM17 / TACE antibody at 10 μg/ml dilution.