ARG10772

anti-ADCY6 antibody

anti-ADCY6 antibody for Confocal microscopy (Confocal),ELISA,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Human,Rat,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes ADCY6
Tested Reactivity Hu, Ms, Rat
Tested Application Confocal, ELISA, ICC/IF, IHC, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ADCY6
Antigen Species Rat
Immunogen KLH-conjugated synthetic peptide around aa. 13-27 of Rat ADCY6. (DERKTAWGERNGQKR)
Conjugation Un-conjugated
Full Name adenylate cyclase 6
Alternate Names Ca; LCCS8; ATP pyrophosphate-lyase 6; Adenylate cyclase type VI; AC6; Adenylate cyclase type 6; EC 4.6.1.1; Adenylyl cyclase 6; 2+

Application Instructions

Application Suggestion
Tested Application Dilution
Confocal1:100 - 1:200
ELISA1:10000
ICC/IF1:100 - 1:200
IHC1:100 - 1:200
IPAssay-dependent
WB1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 131 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 112 Human ADCY6

GeneID: 11512 Mouse ADCY6

GeneID: 25289 Rat ADCY6

Gene Symbol Adcy6
Gene Full Name adenylate cyclase 6
Background This gene encodes a member of the adenylyl cyclase family of proteins, which are required for the synthesis of cyclic AMP. All members of this family have an intracellular N-terminus, a tandem repeat of six transmembrane domains separated by a cytoplasmic loop, and a C-terminal cytoplasmic domain. The two cytoplasmic regions bind ATP and form the catalytic core of the protein. Adenylyl cyclases are important effectors of transmembrane signaling pathways and are regulated by the activity of G protein coupled receptor signaling. This protein belongs to a small subclass of adenylyl cyclase proteins that are functionally related and are inhibited by protein kinase A, calcium ions and nitric oxide. A mutation in this gene is associated with arthrogryposis multiplex congenita. [provided by RefSeq, May 2015]
Function Membrane-bound, calcium-inhibitable adenylyl cyclase. [UniProt]
PTM Phosphorylation by RAF1 increases enzyme activity. Phosphorylation by PKA at Ser-662 inhibits the GNAS-mediated increase in catalytic activity. Phosphorylation by PKC at Ser-556, Ser-662 and Thr-919 inhibits catalytic activity.

Images (3) Click the Picture to Zoom In

  • ARG10772 anti-ADCY6 antibody IHC image

    Immunohistochemistry: Rat heart tissue section stained with ARG10772 anti-ADCY6 antibody at 1:100 dilution.

  • ARG10772 anti-ADCY6 antibody WB image

    Western blot: Recombinant ADCY6 protein stained with ARG10772 anti-ADCY6 antibody at 1:500 dilution.

  • ARG10772 anti-ADCY6 antibody IHC image

    Immunohistochemistry: Rat heart tissue section stained with ARG10772 anti-ADCY6 antibody at 1:100 dilution.