ARG51677

anti-ADD1 phospho (Ser726) antibody

anti-ADD1 phospho (Ser726) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat

Controls and Markers antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes ADD1 phospho (Ser726)
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ADD1
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of serine 726 (T-P-S(p)-F-L) derived from Human ADD1.
Conjugation Un-conjugated
Alternate Names Erythrocyte adducin subunit alpha; Alpha-adducin; ADDA

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11518 Mouse ADD1

GeneID: 118 Human ADD1

GeneID: 24170 Rat ADD1

Gene Symbol ADD1
Gene Full Name adducin 1 (alpha)
Background Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
Function Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. [UniProt]
Research Area Controls and Markers antibody; Signaling Transduction antibody
Calculated MW 81 kDa

Images (3) Click the Picture to Zoom In

  • ARG51677 anti-ADD1 phospho (Ser726) antibody WB image

    Western blot: Extracts from HeLa cells stained with ARG51677 anti-ADD1 phospho (Ser726) antibody and the same antibody preincubated with blocking peptide.

  • ARG51677 anti-ADD1 phospho (Ser726) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung carcinoma tissue stained with ARG51677 anti-ADD1 phospho (Ser726) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51677 anti-ADD1 phospho (Ser726) antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with ARG51677 anti-ADD1 phospho (Ser726) antibody.