ARG57109
anti-ADK antibody [4F8]
anti-ADK antibody [4F8] for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [4F8] recognizes ADK |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4F8 |
| Isotype | IgG1, kappa |
| Target Name | ADK |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 22-362 of Human ADK |
| Conjugation | Un-conjugated |
| Alternate Names | Adenosine kinase; AK; Adenosine 5'-phosphotransferase; EC 2.7.1.20 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ADK |
| Gene Full Name | adenosine kinase |
| Background | This gene an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011] |
| Function | ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides. [UniProt] |
| Calculated MW | 41 kDa |
Images (3) Click the Picture to Zoom In
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ARG57109 anti-ADK antibody [4F8] ICC/IF image
Immunofluorescence: A549 cells line stained with ARG57109 anti-ADK antibody [4F8] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57109 anti-ADK antibody [4F8] FACS image
Flow Cytometry: A549 cell line stained with ARG57109 anti-ADK antibody [4F8] at 2- 5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
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ARG57109 anti-ADK antibody [4F8] WB image
Western blot: 35 µg of HepG2 and 293T cell lysates stained with ARG57109 anti-ADK antibody [4F8] at 1:1000.
