ARG45645
anti-AFF4 antibody
anti-AFF4 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes AFF4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | AFF4 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to N-terminal region of human AFF4. |
| Conjugation | Un-conjugated |
| Alternate Names | AFF4; AF4/FMR2 family, member 4; AF4/FMR2 family member 4; Protein AF-5q31; CHOPS; MCEF; ALL1-fused gene from chromosome 5q31 protein; Major CDK9 elongation factor-associated protein; AF5Q31 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 150 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AFF4 |
| Gene Full Name | AF4/FMR2 family, member 4 |
| Background | The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23). [provided by RefSeq, Oct 2011] |
| Function | Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 127 kDa |
| PTM | Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG45645 anti-AFF4 antibody IHC-P image
Immunohistochemistry: Human placenta stained with ARG45645 anti-AFF4 antibody at 2 μg/ml dilution.
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ARG45645 anti-AFF4 antibody WB image
Western blot: Hela stained with ARG45645 anti-AFF4 antibody at 0.5 μg/ml dilution.
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ARG45645 anti-AFF4 antibody FACS image
Flow Cytometry: SiHa stained with ARG45645 anti-AFF4 antibody at 1 µg/10^6 cells dilution.
