ARG45761
anti-AGO3 / Eif2c3 antibody
anti-AGO3 / Eif2c3 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes AGO3 / Eif2c3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | AGO3 / Eif2c3 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human AGO3 / Eif2c3. |
| Conjugation | Un-conjugated |
| Alternate Names | eIF2C 3; EIF2C3; Argonaute RISC catalytic component 3; Argonaute3; Protein argonaute-3; hAgo3; Eukaryotic translation initiation factor 2C 3; eIF-2C 3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 97 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AGO3 |
| Gene Full Name | argonaute RISC catalytic component 3 |
| Background | This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Function | Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Proposed to be involved in stabilization of small RNA derivates (riRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent riRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. [UniProt] |
| Cellular Localization | Cytoplasm, P-body. [UniProt] |
| Calculated MW | 97 kDa |
| PTM | Acetylation; Phosphoprotein; Ubl conjugation. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG45761 anti-AGO3 / Eif2c3 antibody IHC-P image
Immunohistochemistry: Human cervical carcinoma stained with ARG45761 anti-AGO3 / Eif2c3 antibody at 2 μg/ml dilution.
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ARG45761 anti-AGO3 / Eif2c3 antibody ICC/IF image
Immunofluorescence: U2OS stained with ARG45761 anti-AGO3 / Eif2c3 antibody at 5 μg/ml dilution.
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ARG45761 anti-AGO3 / Eif2c3 antibody WB image
Western blot: K562 stained with ARG45761 anti-AGO3 / Eif2c3 antibody at 0.5 μg/ml dilution.
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ARG45761 anti-AGO3 / Eif2c3 antibody FACS image
Flow Cytometry: Jurkat stained with ARG45761 anti-AGO3 / Eif2c3 antibody at 1 µg/10^6 cells dilution.
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ARG45761 anti-AGO3 / Eif2c3 antibody WB image
Western blot: Rat brain stained with ARG45761 anti-AGO3 / Eif2c3 antibody at 0.5 μg/ml dilution.
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ARG45761 anti-AGO3 / Eif2c3 antibody WB image
Western blot: Mouse brain stained with ARG45761 anti-AGO3 / Eif2c3 antibody at 0.5 μg/ml dilution.
