anti-AGR2 antibody for Western blot,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human
|Product Description||Rabbit Polyclonal antibody recognizes AGR2|
|Tested Application||ICC/IF, IHC-P, WB|
|Immunogen||KLH-conjugated synthetic peptide corresponding to aa. 13-42 (N-terminus) of Human AGR2.|
|Alternate Names||Anterior gradient protein 2 homolog; Secreted cement gland protein XAG-2 homolog; hAG-2; GOB-4; HAG-2; HPC8; PDIA17; HEL-S-116; AG-2; XAG-2; AG2|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Purification||This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Buffer||PBS and 0.09% (W/V) Sodium azide|
|Preservative||0.09% (W/V) Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||anterior gradient 2|
|Function||Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. [UniProt]|
|Cellular Localization||Secreted. Endoplasmic reticulum|
AGR2 antibodies; Anti-Rabbit IgG secondary antibodies;
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|Calculated MW||20 kDa|
Images (3) Click the Picture to Zoom In
ARG55840 anti-AGR2 antibody ICC/IF image
Immunofluorescence: A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then stained with ARG55840 anti-AGR2 antibody (green) at 1:25 dilution, 1 hour at 37°C. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 hour at 37°C). DAPI (blue) for nuclear staining.
ARG55840 anti-AGR2 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung carcinoma tissue stained with ARG55840 anti-AGR2 antibody.
ARG55840 anti-AGR2 antibody WB image
Western blot: 35 µg of HT29 cell lysate stained with ARG55840 anti-AGR2 antibody.