ARG55840

anti-AGR2 antibody

anti-AGR2 antibody for Western blot,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes AGR2
Tested Reactivity Hu
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name AGR2
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 13-42 (N-terminus) of Human AGR2.
Conjugation Un-conjugated
Full Name anterior gradient 2
Alternate Names Anterior gradient protein 2 homolog; Secreted cement gland protein XAG-2 homolog; hAG-2; GOB-4; HAG-2; HPC8; PDIA17; HEL-S-116; AG-2; XAG-2; AG2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:10 - 1:50
IHC-P1:10 - 1:50
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HT29

Properties

Form Liquid
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 10551 Human AGR2

Swiss-port # O95994 Human Anterior gradient protein 2 homolog

Gene Symbol AGR2
Gene Full Name anterior gradient 2
Function Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. [UniProt]
Cellular Localization Secreted. Endoplasmic reticulum
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Images (3) Click the Picture to Zoom In

  • ARG55840 anti-AGR2 antibody ICC/IF image

    Immunofluorescence: A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then stained with ARG55840 anti-AGR2 antibody (green) at 1:25 dilution, 1 hour at 37°C. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 hour at 37°C). DAPI (blue) for nuclear staining.

  • ARG55840 anti-AGR2 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung carcinoma tissue stained with ARG55840 anti-AGR2 antibody.

  • ARG55840 anti-AGR2 antibody WB image

    Western blot: 35 µg of HT29 cell lysate stained with ARG55840 anti-AGR2 antibody.