ARG53743

anti-AGR3 antibody [AGR3.1]

anti-AGR3 antibody [AGR3.1] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human

Cancer antibody

Overview

Product Description

Mouse Monoclonal antibody [AGR3.1] recognizes AGR3

Tested Reactivity Hu
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Specificity The clone AGR3.1 recognizes the epitope HETTDKNLS within the AGR3 (AG3) protein (19-20 kDa); a secreted cytoplasmic protein which can serve as a marker of carcinogenesis.
Host Mouse
Clonality Monoclonal
Clone AGR3.1
Isotype IgG1
Target Name AGR3
Antigen Species Human
Immunogen Purified human AGR3 protein
Conjugation Un-conjugated
Alternate Names Anterior gradient protein 3 homolog; hAG-3; BCMP11; HAG3; AG-3; Breast cancer membrane protein 11; AG3; PDIA18

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1 µg/ml
IHC-Fr1 µg/ml
IHC-P1 µg/ml
WB1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control WB and ICC/IF: Positive control: T47D. Negative control: H1299
IHC-P and IHC-Fr: Positive tissue: breast cancer

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 155465 Human AGR3

Swiss-port # Q8TD06 Human Anterior gradient protein 3

Gene Symbol AGR3
Gene Full Name anterior gradient 3
Background AGR3 (Anterior Gradient 3) protein, also known as AG3 (hAG-3, HAG3 in human), or BCMP11, is a secreted cytoplasmic protein which is involved in metastasis induction and p53 tumour supressor inhibition. It may serve as molecular marker and potential therapeutic target for hormone-responsive breast tumours. Its Xenopus homolog is associated with anteroposterior fate determination during early development.
Research Area Cancer antibody
Calculated MW 19 kDa

Images (2) Click the Picture to Zoom In

  • ARG53743 anti-AGR3 antibody [AGR3.1] WB image

    Western blot: 1. H1229 cell lysate, 2. T47D cell lysate stained with ARG53743 anti-AGR3 antibody [AGR3.1].

  • ARG53743 anti-AGR3 antibody [AGR3.1] IHC-P image

    Immunohistochemistry: Paraffin-embedded sections of breast carcinoma stained with ARG53743 anti-AGR3 antibody [AGR3.1].