ARG11062

anti-AIF1 / Iba 1 antibody

anti-AIF1 / Iba 1 antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Macroglial Marker antibody
publication_link Publication1

Overview

Product Description Rabbit Polyclonal antibody recognizes AIF1 / Iba 1
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-Fr, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name AIF1 / Iba 1
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide around the C-terminal region of Human AIF1 / Iba1.
Conjugation Un-conjugated
Alternate Names Ionized calcium-binding adapter molecule 1; Allograft inflammatory factor 1; IBA1; IRT-1; IRT1; AIF-1; Protein G1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:2000 - 1:5000
IHC-Fr1:2000 - 1:5000
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 17 kDa

Properties

Form Liquid
Purification Unpurified
Buffer Serum and 5mM Sodium azide.
Preservative 5mM Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11629 Mouse AIF1

GeneID: 199 Human AIF1

GeneID: 29427 Rat AIF1

Gene Symbol AIF1
Gene Full Name allograft inflammatory factor 1
Background AIF1 / Iba 1 is a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]
Function AIF1 / Iba 1 is an Actin-binding protein. It enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30324 Neuroinflammation Antibody Panel
Related products:
AIF1 antibodies; AIF1 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Macroglial Marker antibody
Calculated MW 17 kDa
PTM Phosphorylated on serine residues. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG11062 anti-AIF1 / Iba 1 antibody IHC-Fr image

    Immunohistochemistry: High magnification stacked confocal image of Rat cerebellar molecular layer at top and granular layer below, stained with ARG11062 anti-AIF1 / Iba 1 antibody (green) at 1:1000 dilution. Nuclear DNA is shown with DAPI stain in blue.

    Microglia are very small cells with fine processes spreading in three dimensions and so are best visualized in a confocal Z stack. Red shows the processes of Purkinje cells and the perikarya of granule cells revealed by an antibody to MAP2, at 1:5000 dilution.

  • ARG11062 anti-AIF1 / Iba 1 antibody WB image

    Western blot: Mouse brain, Rat brain, Mouse spleen, and Rat spleen lysates stained with ARG11062 anti-AIF1 / Iba 1 antibody at 1:1000 dilution.

    Iba1 is a relatively minor protein of brain and is much more abundant in spleen, so the 15 kDa band is less obvious in CNS lysates. The other bands seen in the CNS lysates are of unknown origin but do not appear to compromise the migroglial specific staining seen with this antibody.

Specific References

Dark Sweet Cherry ( Prunus avium) Phenolics Enriched in Anthocyanins Induced Apoptosis in MDA-MB-453 Breast Cancer Cells through MAPK-Dependent Signaling and Reduced Invasion via Akt and PLCγ-1 Downregulation.

WB / Human

Marjorie Anne A Layosa et al.
Nutr Cancer.,  (2020)

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