anti-AIF1 / Iba 1 antibody
anti-AIF1 / Iba 1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Bovine,Dog,Guinea pig,Human,Marmoset,Mouse,Rabbit,Rat
Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Macroglial Marker antibody
|Product Description||Goat Polyclonal antibody recognizes AIF1 / Iba 1|
|Tested Reactivity||Hu, Ms, Rat, Bov, Dog, Gpig, Marmoset, Rb|
|Predict Reactivity||Macaq, Pig|
|Tested Application||ICC/IF, IHC-P, WB|
|Specificity||This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3). AIF1 / IBA1 is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma.|
|Target Name||AIF1 / Iba 1|
|Alternate Names||Ionized calcium-binding adapter molecule 1; Allograft inflammatory factor 1; IBA1; IRT-1; IRT1; AIF-1; Protein G1|
|Application Note||WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Positive Control||Raw264.7, Mouse brain and Rat brain|
|Observed Size||15 - 18 kDa|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Buffer||Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA|
|Preservative||0.02% Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Background||AIF1 / Iba 1 is a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]|
|Function||AIF1 / Iba 1 is an Actin-binding protein. It enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. [UniProt]|
|Highlight||Related Antibody Duos and Panels:
ARG30317 Activated Macrophage / Microglia Marker Antibody Duo
AIF1 antibodies; AIF1 Duos / Panels; Anti-Goat IgG secondary antibodies;
Microglial help TAM-ing inflammation in the brain
LKB1 deficiency in T cells promotes gut tumors
|Research Area||Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Macroglial Marker antibody|
|Calculated MW||17 kDa|
|PTM||Phosphorylated on serine residues.|
Images (4) Click the Picture to Zoom In
ARG63338 anti-AIF1 / Iba 1 antibody WB image
Western blot: 20 µg of Raw264.7 cell lysate stained with ARG63338 anti-AIF1 / Iba 1 antibody at 1:500 dilution.
ARG63338 anti-AIF1 / Iba 1 antibody ICC/IF image
Immunofluorescence: LPS treated cultured Human microglia stained with ARG63338 anti-AIF1 / Iba 1 antibody at 1:100 dilution.
ARG63338 anti-AIF1 / Iba 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG63338 anti-AIF1 / Iba 1 antibody at 6 µg/ml dilution followed by HRP-staining.
ARG63338 anti-AIF1 / Iba 1 antibody WB image
Western blot: 35 µg of Mouse brain and Rat brain lysates (in RIPA buffer) stained with ARG63338 anti-AIF1 / Iba 1 antibody at 1 µg/ml (left lane) or 2 µg/ml (right lane) and incubated at RT for 1 hour.