ARG45799
anti-AKAP8 / AKAP95 antibody
anti-AKAP8 / AKAP95 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes AKAP8 / AKAP95 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | AKAP8 / AKAP95 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human AKAP8 / AKAP95 . |
| Conjugation | Un-conjugated |
| Alternate Names | AKAP8; A-Kinase Anchoring Protein 8; AKAP-8; AKAP95; AKAP 95; A-kinase anchor protein 95 kDa; AKAP-95; A-kinase anchor protein 8 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 92 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AKAP8 |
| Gene Full Name | A-Kinase Anchoring Protein 8 |
| Background | This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011] |
| Function | Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). [UniProt] |
| Cellular Localization | Cytoplasm; Nucleus. [UniProt] |
| Calculated MW | 76 kDa |
| PTM | Phosphorylated on tyrosine residues probably by SRC subfamily protein kinases; multiple phosphorylation is leading to dissociation from nuclear structures implicated in chromatin structural changes. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG45799 anti-AKAP8 / AKAP95 antibody IHC-P image
Immunohistochemistry: Human appendiceal adenocarcinoma stained with ARG45799 anti-AKAP8 / AKAP95 antibody at 2 μg/ml dilution.
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ARG45799 anti-AKAP8 / AKAP95 antibody ICC/IF image
Immunofluorescence: MCF-7 stained with ARG45799 anti-AKAP8 / AKAP95 antibody at 5 μg/ml dilution.
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ARG45799 anti-AKAP8 / AKAP95 antibody WB image
Western blot: HEK293 stained with ARG45799 anti-AKAP8 / AKAP95 antibody at 0.5 μg/ml dilution.
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ARG45799 anti-AKAP8 / AKAP95 antibody FACS image
Flow Cytometry: A431 stained with ARG45799 anti-AKAP8 / AKAP95 antibody at 1 µg/10^6 cells dilution.
