ARG10692

anti-ALDH1L1 antibody

anti-ALDH1L1 antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow,Horse,Pig

Overview

Product Description Rabbit Polyclonal antibody recognizes ALDH1L1
Tested Reactivity Hu, Ms, Rat, Cow, Hrs, Pig
Predict Reactivity Chk
Tested Application ICC/IF, IHC-Fr, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ALDH1L1
Antigen Species Human
Immunogen Full length Human ALDH1L1 protein.
Conjugation Un-conjugated
Full Name aldehyde dehydrogenase 1 family, member L1
Alternate Names 10-fTHF; EC 1.5.1.6; FDH; Aldehyde dehydrogenase family 1 member L1; FTHFD; 10-FTHFDH; Cytosolic 10-formyltetrahydrofolate dehydrogenase

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:2000
IHC-Fr1:2000
WB1:5000 - 1:10000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Unpurified.
Buffer Serum.
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 107747 Mouse ALDH1L1

GeneID: 10840 Human ALDH1L1

GeneID: 64392 Rat ALDH1L1

Gene Symbol ALDH1L1
Gene Full Name aldehyde dehydrogenase 1 family, member L1
Background The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family. Loss of function or expression of this gene is associated with decreased apoptosis, increased cell motility, and cancer progression. There is an antisense transcript that overlaps on the opposite strand with this gene locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]

Images (5) Click the Picture to Zoom In

  • ARG10692 anti-ALDH1L1 antibody ICC/IF image

    Immunocytochemistry: Rat mixed neuron-glial cell mixture cultures stained with ARG10692 anti-ALDH1L1 antibody (red) and co-stained with monoclonal 5C10 against GFAP (green). ARG10692 anti-ALDH1L1 antibody stains astrocytes and excludes from neuron cells. ALDH1L1 stains the astrocytes cell body and processes, whereas GFAP labels the intermediate filament of the cytoskeleton in subset of astrocytes. Astrocytes are positive for both ALDH1L1 and GFAP appear yellow. ALDH1L1 also labels many astrocytes not labelled by GFAP, which appear as red.

  • ARG10692 anti-ALDH1L1 antibody ICC/IF image

    Immunofluorescence: Cortical neuron-glial cells from E20 Rat stained with ARG10692 anti-ALDH1L1 antibody (red) at 1:1000 dilution and costained with ARG52313 anti-GFAP antibody (green) at 1:5000 dilution. DAPI (blue) for nuclear staining.

    The ALDH1L1 antibody labels protein expressed in the cytoplasm of non-neuronal cells. The GFAP antibody stains intermediate filaments in astrocytes and other glial cells.

  • ARG10692 anti-ALDH1L1 antibody IHC-Fr image

    Immunohistochemistry: Frozen sections of Mouse brain (fixed by transcardial perfusion of 4% paraformaldehyde) stained with ARG10692 anti-ALDH1L1 antibody (green) and Clone 5C10 (red). Along the corpus callosum (white matter), ARG10692 anti-ALDH1L1 antibody labels astrocytes that also heavily express GFAP. As a result, astrocytes in this area appear to be gold to yellow. Inset shows a magnified image. Nuclei are labeled with DAPI (blue).

  • ARG10692 anti-ALDH1L1 antibody WB image

    Western blot: Rat liver tissue homogenates stained with ARG10692 anti-ALDH1L1 antibody.

  • ARG10692 anti-ALDH1L1 antibody WB image

    Western blot: Rat brain, Rat liver and Rat kidney lysates stained with ARG10692 anti-ALDH1L1 antibody (green) at 1:5000 dilution.