ARG51172

anti-AMPK alpha 1/2 antibody

anti-AMPK alpha 1/2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; AMPK-ACC pathway antibody
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Overview

Product Description Rabbit Polyclonal antibody recognizes AMPK alpha 1/2
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name AMPK alpha 1/2
Antigen Species Human
Immunogen Peptide sequence around aa. 172~176/170~174 (L-R-T-S-C) derived from Human AMPKα1/AMPKα2.
Conjugation Un-conjugated
Alternate Names AMPK; Acetyl-CoA carboxylase kinase; ACACA kinase; 5'-AMP-activated protein kinase catalytic subunit alpha-2; EC 2.7.11.31; EC 2.7.11.27; HMGCR kinase; PRKAA; AMPK2; EC 2.7.11.1; AMPK subunit alpha-2; AMPKa2; Hydroxymethylglutaryl-CoA reductase kinase

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 108079 Mouse PRKAA2

GeneID: 5563 Human PRKAA2

GeneID: 78975 Rat PRKAA2

Gene Symbol PRKAA2
Gene Full Name protein kinase, AMP-activated, alpha 2 catalytic subunit
Background Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
Function Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30182 AMPK-ACC pathway Antibody Panel
Related products:
AMPK alpha antibodies; AMPK alpha Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; AMPK-ACC pathway antibody
Calculated MW 62 kDa
PTM Ubiquitinated.
Phosphorylated at Thr-172 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Also phosphorylated at Thr-172 by CAMKK2; triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. CAMKK1 can also phosphorylate Thr-172, but at much lower level. Dephosphorylated by protein phosphatase 2A and 2C (PP2A and PP2C). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK. Dephosphorylated by PPM1A and PPM1B at Thr-172 (mediated by STK11/LKB1).

Images (3) Click the Picture to Zoom In

  • ARG51172 anti-AMPK alpha 1/2 antibody WB image

    Western blot: 30 µg of A549, HepG2, and Mouse liver cell line lysates stained with ARG51172 anti-AMPK alpha 1/2 antibody at 1:500 dilution.

  • ARG51172 anti-AMPK alpha 1/2 antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with ARG51172 anti-AMPK alpha 1/2 antibody.

  • ARG51172 anti-AMPK alpha 1/2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51172 anti-AMPK alpha 1/2 antibody (left) or the same antibody preincubated with blocking peptide (right).

Customer's Feedback

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Review for anti-AMPK alpha 1/2 antibody

Application:WB

Sample:A549

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

The role of CD36-Fabp4-PPARγ in skeletal muscle involves insulin resistance in intrauterine growth retardation mice with catch-up growth

WB / Mouse

Jing Liu et al.
BMC Endocr Disord.,  (2022)

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Berberine improves ovulation and endometrial receptivity in polycystic ovary syndrome.

WB / Human

Zhi Wang et al.
Phytomedicine.,  (2021)

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