ARG45998
anti-ANKLE2 antibody
anti-ANKLE2 antibody for Western blot and Human,Mouse,Rat
Overview
| Product Description | Chicken Polyclonal antibody recognizes ANKLE2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Host | Chicken |
| Clonality | Polyclonal |
| Isotype | IgY |
| Target Name | ANKLE2 |
| Antigen Species | Human |
| Immunogen | A 19 amino acid synthetic peptide within aa. 280 - 330 of human ANKLE2. |
| Conjugation | Un-conjugated |
| Alternate Names | ankyrin repeat and LEM domain containing 2; ANKLE2; Lem4; LEMD7; KIAA0692; LEM4; Ankyrin repeat and LEM domain-containing protein 2; LEM domain-containing protein 4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Observed Size | 121 kDa |
Properties
| Purification | Affinity chromatography purified |
|---|---|
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -48°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ANKLE2 |
| Gene Full Name | ankyrin repeat and LEM domain containing 2 |
| Background | This gene encodes a member of the LEM family of inner nuclear membrane proteins. The encoded protein functions as a mitotic regulator through postmitotic formation of the nuclear envelope. Mutations in this gene cause morphology defects in the nuclear envelope and BAF hyperphosphorylation. [provided by RefSeq, Mar 2014] |
| Function | Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit (PubMed:22770216). Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly (PubMed:22770216). May regulate nuclear localization of VRK1 in non-dividing cells (PubMed:31735666). It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex (PubMed:22770216). Involved in brain development (PubMed:25259927). [UniProt] |
| Cellular Localization | Endoplasmic reticulum membrane. [UniProt] |
| Calculated MW | 121,139,145 kDa |
| PTM | Phosphoprotein. [UniProt] |
