ARG46000

anti-AP3B2 antibody

anti-AP3B2 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes AP3B2
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name AP3B2
Antigen Species Human
Immunogen A 18 amino acid synthetic peptide within the first 50 amino acids of human AP3B2.
Conjugation Un-conjugated
Alternate Names adaptor-related protein complex 3, beta 2 subunit; AP3B2; NAPTB; AP-3 complex subunit beta-2; Adapter-related protein complex 3 subunit beta-2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 128 kDa

Properties

Purification Affinity chromatography purified
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -50°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11775 Mouse AP3B2

GeneID: 8120 Human AP3B2

Swiss-port # Q13367 Human AP-3 complex subunit beta-2

Swiss-port # Q9JME5 Mouse AP-3 complex subunit beta-2

Gene Symbol AP3B2
Gene Full Name adaptor-related protein complex 3, beta 2 subunit
Background Adaptor protein complex 3 (AP-3 complex) is a heterotrimeric protein complex involved in the formation of clathrin-coated synaptic vesicles. The protein encoded by this gene represents the beta subunit of the neuron-specific AP-3 complex and was first identified as the target antigen in human paraneoplastic neurologic disorders. The encoded subunit binds clathrin and is phosphorylated by a casein kinase-like protein, which mediates synaptic vesicle coat assembly. Defects in this gene are a cause of early-onset epileptic encephalopathy. [provided by RefSeq, Feb 2017]
Function Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. [UniProt]
Cellular Localization Cytoplasmic vesicle; Golgi apparatus; Membrane. [UniProt]
Calculated MW 121 kDa
PTM Phosphoprotein. [UniProt]