ARG46007

anti-APC2 antibody

anti-APC2 antibody for Western blot,ICC/IF and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes APC2
Tested Reactivity Hu, Ms
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name APC2
Antigen Species Human
Immunogen A 18 amino acid synthetic peptide within aa. 240 - 290 of human APC2.
Conjugation Un-conjugated
Alternate Names adenomatosis polyposis coli 2; APC2; APCL; APCL; Adenomatous polyposis coli protein 2; Adenomatous polyposis coli protein-like; APC-like

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Purification Affinity chromatography purified
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -57°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10297 Human APC2

Swiss-port # O95996 Human Adenomatous polyposis coli protein 2

Gene Symbol APC2
Gene Full Name adenomatosis polyposis coli 2
Background This gene encodes a strongly conserved protein that has an N-terminal coiled-coil domain followed by an armadillo domain, five 20-amino acid repeats, and two SAMP domains. This protein promotes the assembly of a multiprotein complex that recruits and phosphorylates the Wnt effector beta-catenin and targets beta-catenin for ubiquitylation and proteasomal degradation. This protein therefore plays a role in the reduction of cytoplasmic levels of beta-catenin which in turn reduces activation of Wnt target genes that play a pivotal role in the pathogenesis of various human cancers. The protein encoded by this gene is closely related to the adenomatous polyposis coli (APC) tumor-suppressor protein and has similar tumor-suppressor effects. This gene also plays a role in actin assembly, cell-cell adhesion, and microtubule network formation through its interaction with cytoskeletal proteins. This gene has its highest expression in the central nervous system and is involved in brain development through cytoskeletal regulation in neurons. Alternative splicing produces multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017]
Function Stabilizes microtubules and may regulate actin fiber dynamics through the activation of Rho family GTPases (PubMed:25753423). May also function in Wnt signaling by promoting the rapid degradation of CTNNB1 (PubMed:10021369, PubMed:11691822, PubMed:9823329). [UniProt]
Cellular Localization Cytoplasm; Cytoskeleton; Golgi apparatus. [UniProt]
Calculated MW 244 kDa
PTM Phosphoprotein. [UniProt]