anti-APRT antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry,ICC/IF and Human
Metabolism antibody; Signaling Transduction antibody
|Product Description||Rabbit Polyclonal antibody recognizes APRT|
|Tested Application||FACS, ICC/IF, IHC-P, WB|
|Immunogen||KLH-conjugated synthetic peptide corresponding to aa. 12-40 (N-terminus) of Human APRT.|
|Alternate Names||Adenine phosphoribosyltransferase; AMP; EC 220.127.116.11; APRTD; APRT|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Purification||Purification with Protein A and immunogen peptide.|
|Buffer||PBS and 0.09% (W/V) Sodium azide|
|Preservative||0.09% (W/V) Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||adenine phosphoribosyltransferase|
|Background||Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]|
|Function||Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. [UniProt]|
|Research Area||Metabolism antibody; Signaling Transduction antibody|
|Calculated MW||20 kDa|
Images (4) Click the Picture to Zoom In
ARG54814 anti-APRT antibody ICC/IF image
Immunofluorescence: A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then stained with ARG54814 anti-APRT antibody (green) at 1:25 dilution, 1 h at 37°C. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C).
ARG54814 anti-APRT antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human hepatocarcinoma stained with ARG54814 anti-APRT antibody.
ARG54814 anti-APRT antibody WB image
Western blot: 35 µg of A2058 cell lysate stained with ARG54814 anti-APRT antibody.
ARG54814 anti-APRT antibody FACS image
Flow Cytometry: HepG2 cells stained with ARG54814 anti-APRT antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.