ARG54814

anti-APRT antibody

anti-APRT antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry,ICC/IF and Human

Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes APRT
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name APRT
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 12-40 (N-terminus) of Human APRT.
Conjugation Un-conjugated
Alternate Names Adenine phosphoribosyltransferase; AMP; EC 2.4.2.7; APRTD; APRT

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
ICC/IF1:10 - 1:50
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control A2058

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 353 Human APRT

Swiss-port # P07741 Human Adenine phosphoribosyltransferase

Gene Symbol APRT
Gene Full Name adenine phosphoribosyltransferase
Background Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. [UniProt]
Cellular Localization Cytoplasm.
Research Area Metabolism antibody; Signaling Transduction antibody
Calculated MW 20 kDa

Images (4) Click the Picture to Zoom In

  • ARG54814 anti-APRT antibody ICC/IF image

    Immunofluorescence: A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then stained with ARG54814 anti-APRT antibody (green) at 1:25 dilution, 1 h at 37°C. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C).

  • ARG54814 anti-APRT antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human hepatocarcinoma stained with ARG54814 anti-APRT antibody.

  • ARG54814 anti-APRT antibody WB image

    Western blot: 35 µg of A2058 cell lysate stained with ARG54814 anti-APRT antibody.

  • ARG54814 anti-APRT antibody FACS image

    Flow Cytometry: HepG2 cells stained with ARG54814 anti-APRT antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.