ARG66211

anti-ASC / TMS1 antibody

anti-ASC / TMS1 antibody for Western blot and Human,Mouse

NLRP3 Inflammasome Study antibody; NLRC4 Inflammasome Study antibody

Overview

Product Description

Rabbit Polyclonal antibody recognizes ASC / TMS1

Tested Reactivity Hu, Ms
Predict Reactivity Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ASC / TMS1
Antigen Species Human
Immunogen Fusion protein of Human ASC / TMS1.
Conjugation Un-conjugated
Alternate Names Apoptosis-associated speck-like protein containing a CARD; TMS; PYD and CARD domain-containing protein; hASC; ASC; TMS-1; TMS1; Caspase recruitment domain-containing protein 5; Target of methylation-induced silencing 1; CARD5

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HL60, A549, Raji and RAW264.7 cells.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol.
Preservative 0.05% Sodium azide
Stabilizer 40% Glycerol
Concentration 1.28 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 29108 Human PYCARD

GeneID: 66824 Mouse PYCARD

Swiss-port # Q9EPB4 Mouse Apoptosis-associated speck-like protein containing a CARD

Swiss-port # Q9ULZ3 Human Apoptosis-associated speck-like protein containing a CARD

Gene Symbol PYCARD
Gene Full Name PYD and CARD domain containing
Background ASC / TMS1 is an adaptor protein. It is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function ASC / TMS1 functions as key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in macrophage pyroptosis, a caspase-1-dependent inflammatory form of cell death and is the major constituent of the ASC pyroptosome which forms upon potassium depletion and rapidly recruits and activates caspase-1. In innate immune response believed to act as an integral adapter in the assembly of the inflammasome which activates caspase-1 leading to processing and secretion of proinflammatory cytokines. The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions. Required for recruitment of caspase-1 to inflammasomes containing certain pattern recognition receptors, such as NLRP2, NLRP3, AIM2 and probably IFI16. In the NLRP1 and NLRC4 inflammasomes seems not be required but facilitates the processing of procaspase-1. In cooperation with NOD2 involved in an inflammasome activated by bacterial muramyl dipeptide leading to caspase-1 activation. May be involved in DDX58-triggered proinflammatory responses and inflammasome activation. Isoform 2 may have a regulating effect on the function as inflammasome adapter. Isoform 3 seems to inhibit inflammasome-mediated maturation of interleukin-1 beta. In collaboration with AIM2 which detects cytosolic double-stranded DNA may also be involved in a caspase-1-independent cell death that involves caspase-8. In adaptive immunity may be involved in maturation of dendritic cells to stimulate T-cell immunity and in cytoskeletal rearrangements coupled to chemotaxis and antigen uptake may be involved in post-transcriptional regulation of the guanine nucleotide exchange factor DOCK2; the latter function is proposed to involve the nuclear form. Also involved in transcriptional activation of cytokines and chemokines independent of the inflammasome; this function may involve AP-1, NF-kappa-B, MAPK and caspase-8 signaling pathways. For regulation of NF-kappa-B activating and inhibiting functions have been reported. Modulates NF-kappa-B induction at the level of the IKK complex by inhibiting kinase activity of CHUK and IKBK. Proposed to compete with RIPK2 for association with CASP1 thereby down-regulating CASP1-mediated RIPK2-dependent NF-kappa-B activation and activating interleukin-1 beta processing. Modulates host resistance to DNA virus infection, probably by inducing the cleavage of and inactivating CGAS in presence of cytoplasmic double-stranded DNA (PubMed:28314590). [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30331 NLRP3 Inflammasome Antibody Panel
ARG30332 NLRC4 Inflammasome Antibody Panel
Related products:
TMS1 antibodies; TMS1 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
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Research Area NLRP3 Inflammasome Study antibody; NLRC4 Inflammasome Study antibody
Calculated MW 22 kDa
PTM Phosphorylated.

Images (1) Click the Picture to Zoom In

  • ARG66211 anti-ASC / TMS1 antibody WB image

    Western blot: 40 µg of HL60, A549, Raji and RAW264.7 cell lysates stained with ARG66211 anti-ASC / TMS1 antibody at 1:500 dilution.