anti-ATF2 phospho (Ser490 / Ser498) antibody
anti-ATF2 phospho (Ser490 / Ser498) antibody for Immunohistochemistry,Western blot and Human
Gene Regulation antibody; Immune System antibody
|Product Description||Rabbit Polyclonal antibody recognizes ATF2 phospho (Ser490 / Ser498)|
|Tested Application||IHC, WB|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser490/498 conjugated to KLH|
|Alternate Names||EC 220.127.116.11; Histone acetyltransferase ATF2; Activating transcription factor 2; cAMP-dependent transcription factor ATF-2; Cyclic AMP-dependent transcription factor ATF-2; CREB2; HB16; CREB-2; Cyclic AMP-responsive element-binding protein 2; cAMP-responsive element-binding protein 2; CRE-BP1; cAMP response element-binding protein CRE-BP1; TREB7|
|Application Note||Specific for ~74k ATF2 protein phosphorylated at Ser490,498. The antibody also recognizes the phosphorylated ~54k splice form of ATF2.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Buffer||10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol|
|Stabilizer||0.1 mg/ml BSA, 50% Glycerol|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||activating transcription factor 2|
|Background||The activating transcription factor ATF2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (Maekawa et al., 1989). ATF2 has been implicated in the transcriptional regulation of a number of genes including cytokines, cell cycle control and apoptosis. Various forms of cellular stress, including inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF2 (Ivanov et al., 2003; Morton et al., 2004). Stress induced ATFdependent transcription is dependent on phosphorylation of ATF (Fuchs et al., 2000); Morton et al., 2004). Serine 490 and serine 498 are novel phosphorylation sites on ATF that have recently been identified. ATF2 is particularly abundant in the brain and the ATF2 family of transcription factors is considered an important substrate of signals upstream of the activation of genes associated with neuronal growth and differentiation (Karin and Hunter, 1995). ATF expression has also been linked to the depression in humans (Laifenfeld et al., 2004).|
|Research Area||Gene Regulation antibody; Immune System antibody|
|Calculated MW||55 kDa|
|PTM||Phosphorylation of Thr-69 by MAPK14 and MAPK11, and at Thr-71 by MAPK1/ERK2, MAPK3/ERK1, MAPK11, MAPK12 and MAPK14 in response to external stimulus like insulin causes increased transcriptional activity. Phosphorylated by PLK3 following hyperosmotic stress. Also phosphorylated and activated by JNK and CaMK4. ATM-mediated phosphorylation at Ser-490 and Ser-498 stimulates its function in DNA damage response. Phosphorylation at Ser-62, Thr-73 and Ser-121 activates its transcriptional activity. Phosphorylation at Thr-69 or Thr-71 enhances its histone acetyltransferase (HAT) activity.|
Images (1) Click the Picture to Zoom In
ARG52231 anti-ATF2 phospho (Ser490 / Ser498) antibody WB image
Western blot: Human melanoma cells incubated with varying doses of the radiometric drug NCS showing phospho-specific immunolabeling of the ATF2 protein phosphorylated at Ser 490, 498 stained with ARG52231 anti-ATF2 phospho (Ser490 / Ser498) antibody.