anti-ATF2 phospho (Thr52) antibody

anti-ATF2 phospho (Thr52) antibody for Western blot and Human

Gene Regulation antibody; Immune System antibody


Product Description Rabbit Polyclonal antibody recognizes ATF2 phospho (Thr52)
Tested Reactivity Hu
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ATF2
Antigen Species Human
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding THr52 conjugated to KLH
Conjugation Un-conjugated
Alternate Names EC; Histone acetyltransferase ATF2; Activating transcription factor 2; cAMP-dependent transcription factor ATF-2; Cyclic AMP-dependent transcription factor ATF-2; CREB2; HB16; CREB-2; Cyclic AMP-responsive element-binding protein 2; cAMP-responsive element-binding protein 2; CRE-BP1; cAMP response element-binding protein CRE-BP1; TREB7

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note Specific for the ~72k ATF2 protein phosphorylated at Thr52. Immunolabeling of the ATF2 band is reduced by treatment of the cells with the PKC catalytic inhibitor Gö6850.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human UACC903 melanoma cells


Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 1386 Human ATF2

Swiss-port # P15336 Human Cyclic AMP-dependent transcription factor ATF-2

Gene Symbol ATF2
Gene Full Name activating transcription factor 2
Background The transcription factor ATF2 is a member of the ATF/CREB family of leucine zipper proteins. In response to stress stimuli, it activates a variety of gene targets that are involved in oncogenesis, and has been correlated with maintenance of a cancer cell phenotype (Vlahopoulos et al., 2008). Inhibiting ATF2 impedes melanoma development and elicits tumor suppressor function (Bhoumik et al., 2008). To act as a tumor suppressor, ATF2 must localize at the mitochondria, and phosphorylation at Thr52 by PKCε regulates this translocation (Lau et al., 2012).
Research Area Gene Regulation antibody; Immune System antibody
Calculated MW 55 kDa
PTM Phosphorylation of Thr-69 by MAPK14 and MAPK11, and at Thr-71 by MAPK1/ERK2, MAPK3/ERK1, MAPK11, MAPK12 and MAPK14 in response to external stimulus like insulin causes increased transcriptional activity. Phosphorylated by PLK3 following hyperosmotic stress. Also phosphorylated and activated by JNK and CaMK4. ATM-mediated phosphorylation at Ser-490 and Ser-498 stimulates its function in DNA damage response. Phosphorylation at Ser-62, Thr-73 and Ser-121 activates its transcriptional activity. Phosphorylation at Thr-69 or Thr-71 enhances its histone acetyltransferase (HAT) activity.