anti-ATG4B antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
|Product Description||Rabbit Polyclonal antibody recognizes ATG4B|
|Tested Application||ICC/IF, IHC-P, WB|
|Immunogen||KLH-conjugated synthetic peptide corresponding to aa. 358-390 (C-terminus) of Human ATG4B.|
|Alternate Names||Cysteine protease ATG4B; hAPG4B; Autophagin-1; APG4B; Autophagy-related protein 4 homolog B; Autophagy-related cysteine endopeptidase 1; EC 3.4.22.-; AUT-like 1 cysteine endopeptidase; AUTL1|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Purification||This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Buffer||PBS and 0.09% (W/V) Sodium azide|
|Preservative||0.09% (W/V) Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||autophagy related 4B, cysteine peptidase|
|Background||Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]|
|Function||Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms. [UniProt]|
|Research Area||Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody|
|Calculated MW||44 kDa|
Images (3) Click the Picture to Zoom In
ARG54818 anti-ATG4B antibody ICC/IF image
Immunofluorescence: U251 cells were treated with Chloroquine (50 µM, 16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then stained with ARG54818 anti-ATG4B antibody (green) at 1:100 dilution, 2 h at room temperature. Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). ATG4B immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
ARG54818 anti-ATG4B antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human cancer tissue stained with ARG54818 anti-ATG4B antibody.
ARG54818 anti-ATG4B antibody WB image
Western blot: HeLa cell lysate stained with ARG54818 anti-ATG4B antibody.