ARG46024
anti-ATG4C antibody
anti-ATG4C antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ATG4C |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ATG4C |
| Antigen Species | Human |
| Immunogen | A 18 amino acid synthetic peptide within the last 50 amino acids of human ATG4C. |
| Conjugation | Un-conjugated |
| Alternate Names | ATG4C; Cysteine protease ATG4C; Autophagin-3; AUT-like 3 cysteine endopeptidase; Autophagy-related protein 4 homolog C; APG4C; APG4-C; Autophagy-related cysteine endopeptidase 3; EC 3.4.22.-; AUTL3; AUTL1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 57 kDa |
Properties
| Purification | Affinity chromatography purified |
|---|---|
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ATG4C |
| Gene Full Name | autophagy related 4C, cysteine peptidase |
| Background | Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq, Jul 2008] |
| Function | Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation (By similarity). Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms. [UniProt] |
| Cellular Localization | Cytoplasm. |
| Calculated MW | 50 kDa |
Images (2) Click the Picture to Zoom In
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ARG46024 anti-ATG4C antibody IHC-P image
Immunohistochemistry: Human lung stained with ARG46024 anti-ATG4C antibody.
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ARG46024 anti-ATG4C antibody WB image
Western blot: A549 stained with ARG46024 anti-ATG4C antibody.
