ARG54819

anti-ATG4C antibody

anti-ATG4C antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes ATG4C
Tested Reactivity Hu, Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ATG4C
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 419-448 (C-terminus) of Human ATG4C.
Conjugation Un-conjugated
Alternate Names Cysteine protease ATG4C; Autophagin-3; AUT-like 3 cysteine endopeptidase; Autophagy-related protein 4 homolog C; APG4C; APG4-C; Autophagy-related cysteine endopeptidase 3; EC 3.4.22.-; AUTL3; AUTL1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse liver

Properties

Form Liquid
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 242557 Mouse ATG4C

GeneID: 84938 Human ATG4C

Swiss-port # Q811C2 Mouse Cysteine protease ATG4C

Swiss-port # Q96DT6 Human Cysteine protease ATG4C

Gene Symbol ATG4C
Gene Full Name autophagy related 4C, cysteine peptidase
Background Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq, Jul 2008]
Function Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation (By similarity). Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms. [UniProt]
Cellular Localization Cytoplasm.
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
Calculated MW 52 kDa

Images (2) Click the Picture to Zoom In

  • ARG54819 anti-ATG4C antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human cancer tissue stained with ARG54819 anti-ATG4C antibody.

  • ARG54819 anti-ATG4C antibody WB image

    Western blot: 35 µg of Mouse liver lysate stained with ARG54819 anti-ATG4C antibody at 1:1000 dilution.