anti-ATG9A antibody

anti-ATG9A antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody


Product Description Rabbit Polyclonal antibody recognizes ATG9A
Tested Reactivity Hu
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ATG9A
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 717-746 (C-terminus) of Human ATG9A.
Conjugation Un-conjugated
Alternate Names APG9L1; MGD3208; APG9-like 1; mATG9; Autophagy-related protein 9A

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control A375


Form Liquid
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 79065 Human ATG9A

Swiss-port # Q7Z3C6 Human Autophagy-related protein 9A

Gene Symbol ATG9A
Gene Full Name autophagy related 9A
Function Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H. [UniProt]
Cellular Localization Cytoplasmic vesicle, autophagosome membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=Under amino acid starvation or rapamycin treatment, redistributes from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool. The starvation- induced redistribution depends on ULK1, ATG13, as well as SH3GLB1
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody
Calculated MW 94 kDa

Images (3) Click the Picture to Zoom In

  • ARG54825 anti-ATG9A antibody ICC/IF image

    Immunofluorescence: U251 cells were treated with Chloroquine (50 µM, 16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then stained with ARG54825 anti-ATG9A antibody (green) at 1:100 dilution, 2 h at room temperature. Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). ATG9A immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

  • ARG54825 anti-ATG9A antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer tissue stained with ARG54825 anti-ATG9A antibody.

  • ARG54825 anti-ATG9A antibody WB image

    Western blot: 35 µg of A375 cell lysate stained with ARG54825 anti-ATG9A antibody.